Top
×
J Cancer Metastasis Treat 2019;5:77.10.20517/2394-4722.2019.35© The Author(s) 2019.
Open AccessOriginal Article

Loss of the Krüppel-like factor 4 tumor suppressor is associated with epithelial-mesenchymal transition in colorectal cancer

1Department of Medicine, Stony Brook University School of Medicine, Stony Brook, NY 11794, USA.

2Department of Pathology, Stony Brook University School of Medicine, Stony Brook, NY 11794, USA.

3Department of Family, Population and Preventive Medicine, Stony Brook, NY 11794, USA.

4Department of Physiology and Biophysics, Stony Brook University School of Medicine, Stony Brook, NY 11794, USA.

#Authors contributed equally.

Correspondence Address: Dr. Vincent W. Yang, Department of Medicine, Stony Brook University School of Medicine, HSC T-16, Rm 020, Stony Brook, NY 11794, USA. E-mail: vincent.yang@stonybrookmedicine.edu

    Science Editor: William P. Schiemann | Copy Editor: Jing-Wen Zhang | Production Editor: Jing Yu
    ...

    © The Author(s) 2019. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, sharing, adaptation, distribution and reproduction in any medium or format, for any purpose, even commercially, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

    Abstract

    Aim: Colorectal cancer (CRC) is the third leading cancer-related cause of death due to its propensity to metastasize. Epithelial-mesenchymal transition (EMT) is a multistep process important for invasion and metastasis of CRC. Krüppel-like factor 4 (KLF4) is a zinc finger transcription factor highly expressed in differentiated cells of the intestinal epithelium. KLF4 has been shown to play a tumor suppressor role during CRC tumorigenesis - its loss accelerates development and progression of cancer. The present study examined the relationship between KLF4 and markers of EMT in CRC.

    Methods: Immunofluorescence staining for KLF4 and EMT markers was performed on archived patient samples after colorectal cancer resection and on colonic tissues of mice with colitis-associated cancer.

    Results: We found that KLF4 expression is lost in tumor sections obtained from CRC patients and in those of mouse colon following azoxymethane and dextran sodium sulfate (AOM/DSS) treatment when compared to their respective normal appearing mucosa. Importantly, in CRC patient tumor sections, we observed a negative correlation between KLF4 levels and mesenchymal markers including TWIST, β-catenin, claudin-1, N-cadherin, and vimentin. Similarly, in tumor tissues from AOM/DSS-treated mice, KLF4 levels were negatively correlated with mesenchymal markers including SNAI2, β-catenin, and vimentin and positively correlated with the epithelial marker E-cadherin.

    Conclusion: These findings suggest that the loss of KLF4 expression is a potentially significant indicator of EMT in CRC.

    Introduction

    Colorectal cancer (CRC) is the third leading cause of the cancer-related deaths often with metastasis to the liver, lung, and bone[1,2]. Epithelial-mesenchymal transition (EMT) is a transdifferentiation process that allows a polarized epithelial cell to assume a mesenchymal cell phenotype, characterized by enhanced migratory and invasiveness capacity, elevated resistance to apoptosis, and increased synthesis of extracellular matrix (ECM) components[3]. During the EMT process, epithelial cells lose apical-basal polarity that is accompanied by reorganization of cytoskeleton and reprogramming of the signaling pathways that allow for an increase in motility and the development of an invasive phenotype. This multistep complex process is characterized by modifications in the expression of a host of transcription factors and specific cell-surface proteins, as well as reorganization and expression of cytoskeletal proteins, and production of enzymes that degrade the ECM[3]. A change in some of these factors, such as upregulation of TWIST, SNAI1, SNAI2, ZEB1, vimentin, and N-cadherin, and downregulation of E-cadherin and tight junction proteins such as ZO-1 is indicative of progression of EMT[4,5]. In CRC, EMT has been strongly associated with the invasive and metastatic phenotype, thereby generating the life-threatening manifestations of metastatic disease cancer. The activation of the EMT program has been suggested to be the critical mechanism for the acquisition of malignant phenotypes by epithelial cancer cells[3].

    Krüppel-like factor 4 (KLF4) belongs to the family of zinc-finger transcriptions factors that play critical roles during development, proliferation, differentiation, and homeostasis, as well as development and progression of many diseases including inflammation and carcinogenesis[6-8]. In the digestive tract, KLF4 is predominantly expressed in differentiated cells of the villus and surface epithelium of the small intestine and colon, respectively[9-13]. Importantly, evidence indicates that KLF4 functions as a tumor suppressor that inhibits progression of CRC[12]. It has been shown that loss of KLF4 expression is associated with the early stage of CRC development and that KLF4 is a prognostic indicator for CRC survival and recurrence[14,15]. Recently, we demonstrated that KLF4 also plays a protective role against tumor formation during inflammation-induced colorectal tumorigenesis[16-18]. The biochemical mechanisms triggering the acquisition of the invasive phenotype and the subsequent systemic spread of the cancer cell have been areas of intensive research. Here, we demonstrate that KLF4’s role in colorectal tumorigenesis extends to its ability to regulate EMT.

    Methods

    Samples from patients

    Surgical specimens of resected colorectal cancer specimen obtained from Stony Brook University and State University of New York Downstate were used in this study. In total, 12 specimens were processed for immunofluorescence. All samples were of Caucasian origin, with 2 female and 10 male. One sample was qualified as stage I, one as stage 2, two as stage 3, and eight as stage IV [Table 1]. The protocol for the sample collection was originally approved by the Institutional Review Board by the State University of New York at Stony Brook on 17 October 2014 (CORIHS 2014-2821-F) and qualified for a waiver under the Federal Law of Department of Health and Human Services per article 45CFR46.116.d.

    Table 1

    The characteristics of human samples

    Sample IDAge  SexTissue type/areaStageEthnicity
    SB-03664Male      NSC TSC  IVBCaucasian
    SB-12241Male      NR TR  IVCaucasian
    SB-12472Male      NAC TAC  IVCaucasian
    SB-13072Female      NSC TSC  IVCaucasian
    SB-26352Male      NCE TCE  IVCaucasian
    SB-37836Male      NCE TCE  IVCaucasian
    SB-47485Female      NAC TAC  IVCaucasian
    SB-33775Male      NCE TCE  IIICCaucasian
    SB-39651Male      NSC TSC  IIIACaucasian
    SB-55540Male      NCE TCE  IVCaucasian
    SB-64538Male      NSC TSC  ICaucasian
    SB-67065Male      NSC TSC  IIACaucasian

    Mice

    All animal studies were approved by the Stony Brook University Institutional Animal Care and Use Committee and performed in accordance with institutional policies and NIH guidelines. Mice with the floxed Klf4 gene (Klf4fl/fl) were described previously[12]. These mice were derived from a C57BL/6 background and are indistinguishable from the wild-type mice.

    Azoxymethane and dextran sodium sulfate treatment

    Azoxymethane (AOM) and dextran sodium sulfate (DSS) treatment was performed as described previously[18]. Briefly, adult gender- and age-matched Klf4fl/fl mice (n = 16) were injected intraperitoneally with 10 mg/kg of AOM working solution. After seven days, normal water was replaced with 2.5% DSS in the drinking water for five days, followed by two weeks of recovery (with normal water). This was followed by a second cycle of 2.5% DSS for five days, with two weeks of recovery (with normal water). The mice were euthanized at the end of the last recovery treatment, and samples were collected for pathologic analysis.

    Tissue harvesting and tumor assessment, preparation, and immunostaining

    Tissues were collected and prepared for immunofluorescence as described previously[18]. Briefly, tissue sections were baked in a 65 °C oven overnight, deparaffinized in xylene, and rehydrated by incubation in a decreasing ethanol bath series (100%, 95%, and 70%), followed by antigen retrieval in citrate buffer solution (10 mM sodium citrate and 0.05% Tween-20, pH 6.0) at 120 °C for 10 min using a decloaking chamber (Biocare Medical) and 30 min incubation at 4 °C. The histological sections were incubated with blocking buffer (5% bovine serum albumin and 0.01% Tween 20 in 1 × Tris-buffered phosphate-buffered saline) for 1 h at 37 °C. The primary antibodies goat anti-KLF4 (1:200 for human sections and 1:300 for mice sections; R&D: AF3158), mouse anti-PanCK (1:200 for human sections; Biocare Medical: AE1/AE3), rabbit anti-β-catenin (1:500 for human sections and 1:150 for mice sections; Cell Signaling: 8480), rabbit anti-TWIST (1:500; Abcam: ab49254), rabbit anti-Claudin-1 (1:500; Cell Signaling: 13255), rabbit anti-N-cadherin (1:500; Cell Signaling: 13116), rabbit anti-E-cadherin (1:300 for mice sections, Cell Signaling: 3195), rabbit anti-Vimentin (1:500 for human sections and 1:100 for mice sections; Cell Signaling: 5741), and rabbit anti-SNAI2 (1:500 for human sections and 1:400 for mice sections, Cell Signaling: 9585) were added and incubated at 4 °C overnight. For KLF4, secondary unconjugated bovine anti-goat antibody was added at 1:500 dilution in blocking buffer for 30 min at 37 °C. For human sections to stain for PanCK, secondary unconjugated chicken anti-mouse antibody was added at 1:500 dilution in blocking buffer for 30 min at 37 °C. Appropriate Alexa Fluor-labeled antibodies (Molecular Probes) were added at 1:500 dilution in blocking buffer for 30 min at 37 °C. For mice sections, mouse anti-PanCK antibodies conjugated with Alexa 488 (1:100; ThermoFisher Scientific: 53-9003-82) were used. All slides were counterstained with Hoechst 33258 (ThermoFisher Scientific: H3569) and mounted with Fluoromount Aqueous Mounting Medium (Sigma-Aldrich: F4680). Slides were analyzed using a Nikon eclipse 90i microscope (Nikon Instruments Inc.) equipped with DS-Qi1Mc and DS-Fi1 CCD cameras (Nikon Instruments Inc.).

    Immunofluorescence quantification

    For each EMT/KLF4 co-stain, we quantified slides of 4-6 human specimen. For normal adjacent mucosa and tumor sections, we quantified four separate fields and counted 250-400 cells per each field. Each cell was labeled as positive or negative for KLF4 and positive or negative for each of the specific EMT markers. The statistical analysis was performed using a two-tailed Student t test.

    Cell culture

    SW480 colorectal cancer cell line (ATCC® CCL-228) was maintained in RPMI640 medium supplemented with 10% FBS and 1% penicillin/streptomycin at 37 °C in atmosphere containing 5% CO2. To overexpress Klf4-GFP and GFP-control in SW480 cell line, cells were transiently transfected with 3 μg plasmid DNA (per well in a six-well plate) using Lipofectamine 2000 reagent (ThermoFisher Scientific) according to manufacturer’s instructions. The cell lysates were collected using Laemmli buffer and subjected to Western blot analysis with the following antibodies: rabbit anti-KLF4 (MBL: PM057), rabbit anti-ZEB1 (Cell Signaling: 3396), rabbit anti-SNAI1 (Cell Signaling: 3879), rabbit anti-SNAI2 (Cell Signaling: 9585), and mouse anti-actin (SigmaAldrich: A1978). Then, they were developed using secondary antibodies goat anti-rabbit HRP-conjugated (JacksonImmuno Research: 111-035-144) and goat anti-mouse HRP conjugated (SigmaAldrich: AP200P), respectively.

    Statistical analysis

    Student’s paired or unpaired t test was used for statistical analyses. Differences between values were considered significant when P < 0.05. This analysis was performed using GraphPad Prism version 5.00 for Windows (GraphPad Software, San Diego, CA).

    Results

    Expression of KLF4 in human colorectal cancer is negatively correlated with markers of EMT

    EMT is a precisely orchestrated multistep process regulated by several transcriptional factors including TWIST, SNAI1, SNAI2, and ZEB1[3]. To determine the correlation between KLF4 expression and EMT in CRC, we performed immunofluorescence analysis of matched pairs of archived samples from patients after tumor resections. Firstly, we analyzed the expression pattern of KLF4 and TWIST. As shown in Figure 1A (SB396N), KLF4 is expressed in the nucleus of epithelial cells in the normal-appearing mucosa adjacent to the cancer tissues. These cells are positive for PanCK, an epithelial marker, and negative for the nuclear expression of the biomarker of EMT, TWIST. In contrast, in the tumor samples from the same patient [Figure 1A, SB396T], the expression of KLF4 is downregulated in the epithelial cells, which is accompanied by a significant increase in the expression of TWIST. Our statistical analysis showed that there is a negative correlation between KLF4 and TWIST expression in normal-appearing mucosa and tumor tissues (P < 0.001). Several common signaling pathways regulate factors involved in EMT including HH, WNT, NOTCH, and TGF-β[19]. WNT signaling plays an important role in the homeostasis of the intestinal epithelium and its deregulation leads to cancer formation which is accompanied by modification of the pattern and level of expression of its major effector, β-catenin[20-22]. In the normal-appearing mucosa, β-catenin is predominantly localized to the membrane of the epithelial cells with a modest nuclear staining [Figure 1B, SB474N]. Upon loss of KLF4 in the colorectal tumor [Figure 1B, SB474T], the levels of the cytoplasmic and nuclear β-catenin are significantly increased while its membranous expression is decreased (P < 0.001). Loss of cell polarity and cell-cell junctions is another hallmark of EMT and is characterized by increased expression of claudin-1 and N-cadherin[23-27]. Immunofluorescence staining of claudin-1 [Figure 2A, SB474N] and N-cadherin [Figure 2B, SB378N] within the normal-appearing mucosa shows little or no staining while there is pronounced nuclear KLF4 staining. In matching colorectal cancer tumor tissues with lack of KLF4 expression, both claudin-1 and N-cadherin levels are significantly increased [Figure 2A, SB474T and Figure 2B, SB378T, respectively]. Our analysis showed that there is a significant negative correlation between expression of KLF4 and claudin-1 and N-cadherin between the normal-appearing mucosa and tumor tissues (P < 0.001). Furthermore, staining for vimentin, another mesenchymal marker, showed a lack of expression within the epithelial component of the normal-appearing mucosa [Figure 3, SB474N], but a slight increase within the epithelial compartment upon loss of KLF4 in the tumor tissues [Figure 3, SB474T, white arrowheads]. Statistical analysis showed that the expression levels of KLF4 and vimentin are negatively correlated in normal mucosa (P < 0.05) and in tumor sections (P < 0.001).

    Figure 1. Immunofluorescence staining for PanCK, KLF4, TWIST, and β-catenin in human colonic tissues. Representative images of normal adjacent mucosa and tumor sections from two different human specimens: (A) TWIST (SB396N and SB396T); and (B) β-catenin (SB474N and SB474T). White arrowheads indicate KLF4 stain in stromal tissue. White boxes mark insets. Scale bar: 50 μm

    Figure 2. Immunofluorescence staining for PanCK, KLF4, claudin-1, and N-cadherin in human colonic tissues. Representative images of normal adjacent mucosa and tumor sections from two different human specimens: (A) claudin-1 (SB474N and SB474T); and (B) N-cadherin (SB378N and SB378T). White boxes mark insets. Scale bar: 50 μm

    Figure 3. Immunofluorescence staining for PanCK, KLF4, and vimentin in human colonic tissues. Representative images of normal adjacent mucosa (SB474N) show that KLF4 and vimentin do not co-localize (top inset) and tumor sections (SB474T) that show loss of KLF4 and stain of vimentin in epithelial cells. White boxes mark insets. White arrowheads indicate vimentin stain within epithelial cells (bottom inset). Scale bar: 50 μm

    KLF4 expression is negatively correlated with markers of EMT in a mouse model of colitis-associated cancer

    Recently, in a mouse model, we demonstrated that KLF4 plays a protective role against progression of colitis-associated cancer and that decrease in KLF4 levels is associated with increased aggressiveness of the disease[18]. To test if KLF4 expression levels correlate with the expression pattern of EMT markers, we performed immunofluorescence staining of KLF4 and select EMT markers on the mouse tissues (Klf4fl/fl mice) after treatment with AOM/DSS, as described in the Material and Methods Section. Immunofluorescence staining showed that in normal mucosa KLF4 is expressed in the nuclei of epithelial cells defined by staining for PanCK. Co-staining for SNAI2 showed that SNAI2 was predominantly expressed in the stromal section of the normal-appearing mucosa and tumor section of mice after AOM/DSS treatment [Figure 4A] and was absent from epithelial compartment. However, in the tumor section where KLF4 loss was observed, we noticed increased levels of SNAI2 in the nuclei of the epithelial cells that were defined by positive staining for PanCK [Figure 4A]. As in human specimens, we performed immunostaining analysis for β-catenin. However, we did not observe an increase in nuclear β-catenin staining in the tumor sections in comparison to the normal adjacent mucosa [Figure 4B]. With respect to adherens junction complexes, we observed a reduction in the expression levels of E-cadherin in the tumor section as compared to the normal adjacent mucosa [Figure 5A]. Expression of the mesenchymal marker vimentin in normal adjacent mucosa of mice after AOM/DSS treatment is confined to the stroma [Figure 5B] and does not correlate with KLF4 expression. However, in the tumor of mice after AOM/DSS treatment, we observed a slight increase in the staining of vimentin within the epithelial section, which suggests a change in the characteristics of these cells from epithelial toward mesenchymal phenotype [Figure 5B]. Furthermore, overexpression of KLF4 in SW480 CRC cell line resulted in decreased levels of the mesenchymal markers of EMT, namely ZEB1, SNAI1, and SNAI2 [Figure 6], confirming a suppressive role in the regulation of the epithelial-to-mesenchymal transition.

    Figure 4. Immunofluorescence staining for PanCK, KLF4, SNAI2 and, β-catenin in mouse colonic tissues after AOM/DSS treatment. (A) Representative images of PanCK, KLF4, and SNAI2 in normal adjacent mucosa (top panel) and tumor sections (bottom panel). (B) Representative images of PanCK, KLF4, and β-catenin in normal adjacent mucosa (top panel) and tumor sections (bottom panel). Scale bar: 50 μm. AOM/DSS: azoxymethane and dextran sodium sulfate

    Figure 5. Immunofluorescence staining for PanCK, KLF4, E-cadherin, and vimentin in mouse colonic tissues after AOM/DSS treatment. (A) Representative images of PanCK, KLF4, and E-cadherin in normal adjacent mucosa (top panel) and tumor sections (bottom panel). (B) Representative images of PanCK, KLF4, and vimentin in normal adjacent mucosa (top panel) and tumor sections (bottom panel). Dotted area shows transition from epithelial (PanCK staining) to mesenchymal characteristic (vimentin staining). Scale bar: 50 μm. AOM/DSS: azoxymethane and dextran sodium sulfate

    Figure 6. Overexpression of KLF4 in CRC cell line decreases the levels of mesenchymal EMT markers. Western blot analysis of KLF4 and EMT markers ZEB1, SNAI1, and SNAI2 in SW480 colon cancer cell line with EGFP or KLF4-EGFP overexpression. Actin is a loading control. EMT: epithelial-mesenchymal transition

    Discussion

    In this study, we investigated the correlation in expression between KLF4 and EMT markers in tissues obtained from patients with CRC and from a mouse model of colitis-associated cancer. The results from studies of epidermal cancer, hepatocellular carcinoma, breast cancer, pancreatic cancer, and prostate cancer with data predominantly originating from in vitro experiments show that KLF4 negatively regulates EMT[28-33]. On the other hand, KLF4’s ability to regulate the stemness of cancer cells has been shown as an important factor in stimulating EMT in pancreatic, ovarian, endometrial, nasopharyngeal, prostate, and non-small cell lung cancers[34-40]. We demonstrated that KLF4 expression is positively correlated with epithelial markers of EMT in normal mucosa and negatively with mesenchymal markers in CRC. These results are in agreement with previous observations that KLF4 is a suppressor of EMT[28-31,41]. This could be accredited to the role of KLF4 in the regulation of differentiation along the crypt-luminal axis in the intestinal epithelium[10]. Importantly, KLF4’s suppressive role in EMT regulation is not limited to the colonic epithelium but has been shown to play a crucial role in corneal epithelial cell fate. It was shown that downregulation of KLF4 promotes expression of mesenchymal markers and decreases the expression of epithelial markers[42-45]. Furthermore, studies using conditional ablation of KLF4 from the intestinal epithelium showed a deficiency in goblet cell differentiation, thereby demonstrating that KLF4 plays a role in maintaining intestinal epithelial morphology and homeostasis[9,12]. Additionally, KLF4 has been shown to regulate apical-basolateral polarity in intestinal epithelial cells and to enhance their polarity by transcriptional regulation[13]. Thus, loss of KLF4 expression during development and progression of CRC may lead to loss of cell polarity with progression toward EMT. Furthermore, it has been previously demonstrated that E-cadherin (Cdh1), N-cadherin (Cdh2), vimentin (Vim), and β-catenin (Ctnnb1) genes are direct transcriptional targets of KLF4[30]. Yori et al.[29,41] demonstrated that KLF4 is necessary for the maintenance of E-cadherin expression, repression of SNAI1, and prevention of EMT in mammary epithelial cells. These results are consistent with our observations. We demonstrated a positive correlation between KLF4 and E-cadherin and a negative one between KLF4 and N-cadherin and vimentin in human and mouse tissues. However, we were not able to show increased nuclear levels of β-catenin in mouse tissues after AOM/DSS treatment[18]. This could be due to technical differences, as we previously used immunohistochemical staining and the current analysis was based on immunofluorescence studies. In addition, we showed that KLF4 expression is negatively correlated to the expression of TWIST, SNAI2, and claudin-1. Taken together, these results suggest that KLF4 may regulate EMT at several different stages of CRC progression, including suppression of expression of transcription factors (TWIST and SNAI2), transcriptional co-activator (β-catenin), and regulation of cell polarity (E-cadherin, N-cadherin, and claudin-1). In conclusion, we showed a negative association between KLF4 and mesenchymal EMT markers in both human and mouse CRC tissues. Future studies are necessary to identify the mechanism by which KLF4 regulates EMT progression in CRC.

    Declarations

    Acknowledgments

    We thank the Department of Pathology at Stony Brook University for technical assistance in histopathological analysis.

    Authors’ contributions

    Made substantial contributions to conception and design of the study and performed data analysis and interpretation: Yang VW, Ghaleb AM, Bialkowska AB

    Performed data acquisition and analyzed data: Agbo KC, Huang JZ, Ghaleb AM

    Provided tissues samples: Williams JL

    Provided assistance with histopathological analysis: Shroyer KR

    Availability of data and materials

    Not applicable.

    Financial support and sponsorship

    This work was supported by grants from the National Institutes of Health awarded to Yang VW (CA084197).

    Conflicts of interest

    All authors declared that there are no conflicts of interest.

    Ethical approval and consent to participate

    The protocol for the sample collection was originally approved by the Institutional Review Board by the State University of New York at Stony Brook on October 17th, 2014 (CORIHS 2014-2821-F) and qualified for a waiver under the Federal Law of Department of Health and Human Services per article 45CFR46.116.d.

    Consent for publication

    Not applicable.

    Copyright

    © The Author(s) 2019.

    References

    • 1. Engstrand J, Nilsson H, Stromberg C, Jonas E, Freedman J. Colorectal cancer liver metastases - a population-based study on incidence, management and survival. BMC Cancer 2018;18:78.

      DOIPubMedPMC
    • 2. Qiu M, Hu J, Yang D, Cosgrove DP, Xu R. Pattern of distant metastases in colorectal cancer: a SEER based study. Oncotarget 2015;6:38658-66.

      DOIPubMedPMC
    • 3. Lamouille S, Xu J, Derynck R. Molecular mechanisms of epithelial-mesenchymal transition. Nat Rev Mol Cell Biol 2014;15:178-96.

      DOIPubMedPMC
    • 4. Zhu QC, Gao RY, Wu W, Qin HL. Epithelial-mesenchymal transition and its role in the pathogenesis of colorectal cancer. Asian Pac J Cancer Prev 2013;14:2689-98.

      DOIPubMed
    • 5. Thiery JP, Acloque H, Huang RY, Nieto MA. Epithelial-mesenchymal transitions in development and disease. Cell 2009;139:871-90.

      DOIPubMed
    • 6. McConnell BB, Ghaleb AM, Nandan MO, Yang VW. The diverse functions of Kruppel-like factors 4 and 5 in epithelial biology and pathobiology. Bioessays 2007;29:549-57.

      DOIPubMedPMC
    • 7. Evans PM, Liu C. Roles of Krupel-like factor 4 in normal homeostasis, cancer and stem cells. Acta Biochim Biophys Sin (Shanghai) 2008;40:554-64.

      DOIPubMedPMC
    • 8. Ghaleb AM, Yang VW. Kruppel-like factor 4 (KLF4): what we currently know. Gene 2017;611:27-37.

      DOIPubMedPMC
    • 9. Katz JP, Perreault N, Goldstein BG, Lee CS, Labosky PA, et al. The zinc-finger transcription factor Klf4 is required for terminal differentiation of goblet cells in the colon. Development 2002;129:2619-28.

      PubMedPMC
    • 10. Flandez M, Guilmeau S, Blache P, Augenlicht LH. KLF4 regulation in intestinal epithelial cell maturation. Exp Cell Res 2008;314:3712-23.

      DOIPubMedPMC
    • 11. Zheng H, Pritchard DM, Yang X, Bennett E, Liu G, et al. KLF4 gene expression is inhibited by the notch signaling pathway that controls goblet cell differentiation in mouse gastrointestinal tract. Am J Physiol Gastrointest Liver Physiol 2009;296:G490-8.

      DOIPubMedPMC
    • 12. Ghaleb AM, McConnell BB, Kaestner KH, Yang VW. Altered intestinal epithelial homeostasis in mice with intestine-specific deletion of the Kruppel-like factor 4 gene. Dev Biol 2011;349:310-20.

      DOIPubMedPMC
    • 13. Yu T, Chen X, Zhang W, Li J, Xu R, et al. Kruppel-like factor 4 regulates intestinal epithelial cell morphology and polarity. PLoS One 2012;7:e32492.

      DOIPubMedPMC
    • 14. Choi BJ, Cho YG, Song JW, Kim CJ, Kim SY, et al. Altered expression of the KLF4 in colorectal cancers. Pathol Res Pract 2006;202:585-9.

      DOIPubMed
    • 15. Patel NV, Ghaleb AM, Nandan MO, Yang VW. Expression of the tumor suppressor Kruppel-like factor 4 as a prognostic predictor for colon cancer. Cancer Epidemiol Biomarkers Prev 2010;19:2631-8.

      DOIPubMedPMC
    • 16. Ghaleb AM, Laroui H, Merlin D, Yang VW. Genetic deletion of Klf4 in the mouse intestinal epithelium ameliorates dextran sodium sulfate-induced colitis by modulating the NF-kappaB pathway inflammatory response. Inflamm Bowel Dis 2014;20:811-20.

      DOIPubMedPMC
    • 17. Ghaleb AM, Elkarim EA, Bialkowska AB, Yang VW. KLF4 suppresses tumor formation in genetic and pharmacological mouse models of colonic tumorigenesis. Mol Cancer Res 2016;14:385-96.

      DOIPubMedPMC
    • 18. Yang VW, Liu Y, Kim J, Shroyer KR, Bialkowska AB. Increased genetic instability and accelerated progression of colitis-associated colorectal cancer through intestinal epithelium-specific deletion of Klf4. Mol Cancer Res 2019;17:165-76.

      DOIPubMedPMC
    • 19. Mittal V. Epithelial mesenchymal transition in tumor metastasis. Annu Rev Pathol 2018;13:395-412.

      DOIPubMed
    • 20. Basu S, Cheriyamundath S, Ben-Ze’ev A. Cell-cell adhesion: linking Wnt/beta-catenin signaling with partial EMT and stemness traits in tumorigenesis. F1000Res 2018;7.

      DOIPubMedPMC
    • 21. Bialkowska AB, Yang VW. High-throughput screening strategies for targeted identification of therapeutic compounds in colorectal cancer. Future Oncol 2012;8:259-72.

      DOIPubMedPMC
    • 22. Ghahhari NM, Babashah S. Interplay between microRNAs and WNT/beta-catenin signalling pathway regulates epithelial-mesenchymal transition in cancer. Eur J Cancer 2015;51:1638-49.

      DOIPubMed
    • 23. Kourtidis A, Lu R, Pence LJ, Anastasiadis PZ. A central role for cadherin signaling in cancer. Exp Cell Res 2017;358:78-85.

      DOIPubMedPMC
    • 24. Yan X, Yan L, Liu S, Shan Z, Tian Y, et al. N-cadherin, a novel prognostic biomarker, drives malignant progression of colorectal cancer. Mol Med Rep 2015;12:2999-3006.

      DOIPubMed
    • 25. Dhawan P, Singh AB, Deane NG, No Y, Shiou SR, et al. Claudin-1 regulates cellular transformation and metastatic behavior in colon cancer. J Clin Invest 2005;115:1765-76.

      DOIPubMedPMC
    • 26. Singh AB, Sharma A, Smith JJ, Krishnan M, Chen X, et al. Claudin-1 up-regulates the repressor ZEB-1 to inhibit E-cadherin expression in colon cancer cells. Gastroenterology 2011;141:2140-53.

      DOIPubMedPMC
    • 27. Bhat AA, Ahmad R, Uppada SB, Singh AB, Dhawan P. Claudin-1 promotes TNF-alpha-induced epithelial-mesenchymal transition and migration in colorectal adenocarcinoma cells. Exp Cell Res 2016;349:119-27.

      DOIPubMedPMC
    • 28. Lin ZS, Chu HC, Yen YC, Lewis BC, Chen YW. Kruppel-like factor 4, a tumor suppressor in hepatocellular carcinoma cells reverts epithelial mesenchymal transition by suppressing slug expression. PLoS One 2012;7:e43593.

      DOIPubMedPMC
    • 29. Yori JL, Seachrist DD, Johnson E, Lozada KL, Abdul-Karim FW, et al. Kruppel-like factor 4 inhibits tumorigenic progression and metastasis in a mouse model of breast cancer. Neoplasia 2011;13:601-10.

      DOIPubMedPMC
    • 30. Tiwari N, Meyer-Schaller N, Arnold P, Antoniadis H, Pachkov M, et al. Klf4 is a transcriptional regulator of genes critical for EMT, including Jnk1 (Mapk8). PLoS One 2013;8:e57329.

      DOIPubMedPMC
    • 31. Koopmansch B, Berx G, Foidart JM, Gilles C, Winkler R. Interplay between KLF4 and ZEB2/SIP1 in the regulation of E-cadherin expression. Biochem Biophys Res Commun 2013;431:652-7.

      DOIPubMed
    • 32. Mistry DS, Chen Y, Wang Y, Zhang K, Sen GL. SNAI2 controls the undifferentiated state of human epidermal progenitor cells. Stem Cells 2014;32:3209-18.

      DOIPubMedPMC
    • 33. Zhu Z, Yu Z, Wang J, Zhou L, Zhang J, et al. Kruppel-like factor 4 inhibits pancreatic cancer epithelial-to-mesenchymal transition and metastasis by down-regulating caveolin-1 expression. Cell Physiol Biochem 2018;46:238-52.

      DOIPubMed
    • 34. Pinho AV, Rooman I, Real FX. p53-dependent regulation of growth, epithelial-mesenchymal transition and stemness in normal pancreatic epithelial cells. Cell Cycle 2011;10:1312-21.

      DOIPubMed
    • 35. Sureban SM, May R, Qu D, Weygant N, Chandrakesan P, et al. DCLK1 regulates pluripotency and angiogenic factors via microRNA-dependent mechanisms in pancreatic cancer. PLoS One 2013;8:e73940.

      DOIPubMedPMC
    • 36. Chen Z, Wang Y, Liu W, Zhao G, Lee S, et al. Doxycycline inducible Kruppel-like factor 4 lentiviral vector mediates mesenchymal to epithelial transition in ovarian cancer cells. PLoS One 2014;9:e105331.

      DOIPubMedPMC
    • 37. Dong P, Kaneuchi M, Watari H, Hamada J, Sudo S, et al. MicroRNA-194 inhibits epithelial to mesenchymal transition of endometrial cancer cells by targeting oncogene BMI-1. Mol Cancer 2011;10:99.

      DOIPubMedPMC
    • 38. Wu A, Luo W, Zhang Q, Yang Z, Zhang G, et al. Aldehyde dehydrogenase 1, a functional marker for identifying cancer stem cells in human nasopharyngeal carcinoma. Cancer Lett 2013;330:181-9.

      DOIPubMed
    • 39. Ren D, Wang M, Guo W, Zhao X, Tu X, et al. Wild-type p53 suppresses the epithelial-mesenchymal transition and stemness in PC-3 prostate cancer cells by modulating miR145. Int J Oncol 2013;42:1473-81.

      DOIPubMed
    • 40. Kumar M, Allison DF, Baranova NN, Wamsley JJ, Katz AJ, et al. NF-kappaB regulates mesenchymal transition for the induction of non-small cell lung cancer initiating cells. PLoS One 2013;8:e68597.

      DOIPubMedPMC
    • 41. Yori JL, Johnson E, Zhou G, Jain MK, Keri RA. Kruppel-like factor 4 inhibits epithelial-to-mesenchymal transition through regulation of E-cadherin gene expression. J Biol Chem 2010;285:16854-63.

      DOIPubMedPMC
    • 42. Delp EE, Swamynathan S, Kao WW, Swamynathan SK. Spatiotemporally regulated ablation of Klf4 in adult mouse corneal epithelial cells results in altered epithelial cell identity and disrupted homeostasis. Invest Ophthalmol Vis Sci 2015;56:3549-58.

      DOIPubMedPMC
    • 43. Tiwari A, Loughner CL, Swamynathan S, Swamynathan SK. KLF4 plays an essential role in corneal epithelial homeostasis by promoting epithelial cell fate and suppressing epithelial-mesenchymal transition. Invest Ophthalmol Vis Sci 2017;58:2785-95.

      DOIPubMedPMC
    • 44. Tiwari A, Swamynathan S, Alexander N, Gnalian J, Tian S, et al. KLF4 regulates corneal epithelial cell cycle progression by suppressing canonical TGF-beta signaling and upregulating CDK inhibitors P16 and P27. Invest Ophthalmol Vis Sci 2019;60:731-40.

      DOIPubMedPMC
    • 45. Fujimoto S, Hayashi R, Hara S, Sasamoto Y, Harrington J, et al. KLF4 prevents epithelial to mesenchymal transition in human corneal epithelial cells via endogenous TGF-beta2 suppression. Regen Ther 2019;11:249-57.

      DOIPubMedPMC

    Article Access Statistics

    • Viewed: 121
    • Downloaded: 58
    • Cited: Crossref0

    See Updates

    Recommended Articles

    Table of Contents