2Department of Molecular Medicine,
3Medical Oncology Unit,
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Glioblastoma multiforme is considered one of the most common malignant primary intracranial tumors. Despite treatment with a combination of surgery, chemotherapy and radiotherapy, patients with glioblastoma multiform have poor prognosis. It has been widely accepted that the occurrence, progression, and even recurrence of glioblastoma multiforme strictly depends on the presence of glioma cancer stem cells. The presence of glioma stem cells reduces the efficacy of standard therapies, thus increasing the imperative to identify new targets and therapeutic strategies in glioblastoma patients. In this regard, the p21Cip1 pathway has been found to play an important role in the maintenance of the glioma stem cells. It has been shown that this pathway regulates cancer stem cell pool by preventing hyperproliferation and exhaustion. MicroRNAs, endogenous small non-coding RNAs, and long non-coding RNAs, regulate post-transcription gene expression. These are not only altered in glioma, but also in other cancer types, and are involved in tumor development and progression. Notably, they have also been shown to modulate the expression of proteins in the p21Cip1 signaling pathway. This review highlights the extent and complexity of cross-talk between microRNAs, long non-coding RNAs and the p21Cip1 pathway, and demonstrates how such interplay orchestrates the regulation of protein expression and functions in glioma and glioma stem cells.
Glioma, microRNA, long non-coding RNA, p21Cip1, glioma stem cells
According to the World Health Organization (WHO) classification, Glioblastoma multiforme (GBM) is a grade IV malignant glial tumor which displays astrocytic differentiation. It is considered one of the most common malignant primary intracranial tumors. Despite treatment with a combination of surgery, chemotherapy and radiotherapy, GBM patients have poor prognosis. In 1940, the terms primary glioblastoma (pGBM) and secondary glioblastoma (sGBM) were first used by Scherer to distinguish between rapidly progressing de novo tumors and tumors derived from a pre-existing astrocytoma. According to Scherer, pGBM and sGBM display the same histomorphological hallmarks, while differing in biological properties such as type of evolution, clinical manifestation and progression. The differences among primary and secondary GBM arise at the genotypic and epigenetic levels. It is currently widely accepted that the occurrence, progression and even recurrence of both pGBM and sGBM, depend on the accumulation of mutations in neural stem cells, which undergo transformation into glioma cancer stem cells (GSCs). GSCs are characterized by self-renewal and asymmetric cell division, thus allowing the production of proliferating progenitor cells with stem cell features and differentiated cancer cells. Importantly, GSCs are more resistant to radio- and chemotherapy respect to the proliferative progenitors present in the tumor. The p21Cip1 pathway plays an important role in the maintenance of the GSC pool because it induces a quiescent state that prevents hyperproliferation and exhaustion. p21Cip1 is involved in cell cycle arrest which occurs in response to DNA damage. It stimulates DNA repair, thus reducing genetic instability. Thus, p21Cip1 is fundamental in maintaining the GSC pool and its genomic integrity.
As the presence of GSCs reduces the efficacy of standard therapy, there is an increased imperative to identify new targets and therapeutic strategies in GBM patients.
The proteins, p21Cip1, p27KIP1 and p57KIP2, belong to the Cip/Kip family of cyclin dependent kinase inhibitors (CKIs) and are found in mammals. Although p21Cip1 and p27KIP1 were initially classified as tumor suppressor proteins due to their involvement in the p53-dependent cell cycle arrest, it is now evident that their roles are more complex. Dysregulation of Cip/Kip protein may provoke specific defects in its tumor suppressor activity, while its oncogenic functions may be preserved, thus supporting cancer development. p21Cip1 acts as a tumor suppressor through binding and inhibiting CDK/cyclin complexes and the proliferating cell nuclear antigen (PCNA), thus blocking cell proliferation. However, it has been shown that p21Cip1 also displays additional functions such as stem cell pool preservation, regulation of cell migration and apoptosis[7,8]. While some cancer types do not express p21Cip1, its over-expression or cytoplasmic localization has been associated with poor prognosis in several malignant tumors[Figure 1].
p21, a potent cyclin-dependent kinase (CDK) inhibitor, also known as p21Cip1/Waf1/CDKN1A, is a small protein consisting of 165 amino acids. p21 is able to arrest the cell cycle in the G1/S and G2/M phases by inhibiting CDK4, 6/cyclin-D and CDK2/cyclin-E, thus controlling E2F activity[8,9]. The principal transcription regulator of p21Cip1 is p53. In fact, DNA damage and oxidative stress enhance p53 activity and trigger p21Cip1 expression. In addition, under the influence of certain factors, the p21Cip1-dependent cell cycle arrest can be also regulated in a p53-independent manner. As such, p21Cip1 can be transactivated by the BRCA1 variants in a p53-dependent and independent manner. Additionally, transforming growth factor β (TGFβ) and mitogen activated protein kinase control p21Cip1 expression by phosphorylation of SMAD1 and SMAD3, while c-Myc over-expression abrogates TGFβ-mediated p21Cip1/SMAD binding. The E3 ubiquitin ligase, Makorin Ring Finger Protein-1 [a protein involved in cell cycle regulation] reduces p21Cip1 levels by p53 ubiquitination and direct p21Cip1 polyubiquitination. Contrarily, Double homeobox4, which enhances p21Cip1 promoter activity via the transcription factor Sp1, increases p21Cip1 expression levels. In addition, promoter activity and p21Cip1 transcription can be stimulated in a p53-independent pathway via over-expression of β1-integrin receptors, and engagement of the transcription factor Sp1 and the P300 co-activator p21Cip1. Down-regulation of p21Cip1 mRNA expression can also be induced by c-Myc through interactions with the initiator binding zinc finger transcription factor, MIZ-1. Similarly, the pro-myelotic zinc finger can bind to the p21Cip1 promoter, thus blocking the Sp1/p21Cip1 promoter interaction and reducing p21Cip1 expression. The liver kinase B1 is a serine/threonine kinase that activates AMPK and regulates cell growth and apoptosis by triggering a p53-dependent p21Cip1 increase[18,19]. Enhanced p21Cip1 levels, induced by increased p53 and Sp1/DNA binding activity, have been also found in human fibroblasts during replicative senescence[20-22]. Moreover, the alkylating agent Temozolomide (TMZ) induces G2-M cell cycle arrest and senescence through the Mre11-Rad50-Nbs1 (MRN) complex and activation of the ATR/CHK1 axis. TMZ-induced senescence requires a functional p53, a functional NF-κB pathway, as well as a sustained p21Cip1 induction. Upon TMZ exposure, as a consequence of the E2F1/DP1 complex disruption, a strong repression of the mismatch repair proteins MSH2, MSH6, EXO1 and RAD51 was observed.
The Wnt signaling pathway is a critical regulator of cancer stem cell (CSC) features and has been associated with poor prognosis. Its downregulation is responsible for diminished numbers of CD133+CD44+ CSCs [in this regard, recent studies on colon cancer have demonstrated that the knock-down of zeste homologue 2 (EZH2), a key component of the Polycomb-Repressive Complex 2, is involved in maintaining the transcriptional repressive state of cycle arrest and apoptosis of CSC-like cells. EZH2 expression positively correlates with levels of Wnt/β-catenin pathway target genes. EZH2 is essential for the maintenance of CSC-like cell properties] [Figure 2]. Several findings demonstrate that EZH2 knockdown inactivates the Wnt/β-catenin pathway by increasing p21Cip1 expression, resulting in G1/S-phase arrest. EZH2 knockdown in CD133+/CD44+ SW480 cells has been shown to induce the increase of p21Cip1 with consequent reduction of β-catenin, vimentin and c-Myc expression. Moreover, the inhibition of EZH2 via a specific inhibitor promotes enhancement of p21Cip1 expression and inactivation of the Wnt/β-catenin pathway.
Figure 2. EZH2/WNT/p21Cip1 pathway in cancer stem cells
Interestingly, the role of EZH2 in chemotherapy drug resistance has also been investigated. A decrease in EZH2 expression was demonstrated to promote apoptosis, cell cycle arrest at the G1/S phase, and reduce expression of multi-drug resistant proteins in TMZ-resistant GBM cells. GSC maintenance involves the cell-division cycle protein 20 (CDC20), an activator of the E3 ubiquitination ligase complex. Silencing CDC20 expression attenuates GSC proliferation, self-renewal and in vivo tumor growth by inducing apoptosis and cell cycle inhibition. CDC20 maintains the GSC phenotype through p21Cip1 degradation. CDC20 inhibition stabilizes p21Cip1 and represses Survivin, CDC25C and c-Myc survival gene expression.
In addition, the roles of p21Cip1 and p27KIP1 in GSCs treated with camptothecin [CPT, a specific inhibitor of the DNA topoisomerase I that induces DNA double-strand breaks in GSCs carrying the homozygous CDKN2A/ARF deletion] have recently been investigated. The results showed that both p21Cip1 and p27KIP1 proteins block the cell cycle in both unstressed conditions and in response to genotoxic stress. Cip/Kips expression was upregulated after CPT treatment, with a peculiarly nuclear localization. CKIs protect cancer cells against CPT damage by stopping cell cycle progression. Notably, cells become more susceptible to DNA damage in the absence of p21Cip1 and p27KIP1 proteins, leading to impaired cell cycle blockage under genotoxic stress. Overall, the existing literature demonstrates that p21Cip1 and p27KIP1 may act both as tumor suppressors by reducing cell proliferation, and as oncogenes, by increasing cellular resistance in response to DNA damage. A deeper understanding of Cip/Kip functions may be relevant and useful to improve understanding on the mechanisms underlying the acquisition of chemoresistance in cancer.
Finally, p21Cip1 is one of the major regulators of cell cycle and has been previously linked to apoptosis resistance in glioma cells. O(6)-Methylguanine-DNA methyltransferase (MGMT) plays a major role in the resistance to alkylating agents in gliomas. Happold et al. demonstrated that in TMZ-resistant glioma cell line, constitutively expressing MGMT, but not in glioma cell lines negative for MGMT, a strong up-regulation of MGMT levels and elevated p21Cip1 mRNA levels and slower cell cycle progression; however, silencing p21Cip1 silencing in resistant vs. normal glioma cells does not evidenced major changes in cell cycle distribution. Mostofa et al. clarified the role for p21Cip1 in TMZ-resistance in glioma cells-the p21Cip1 protein sequestrates PCNA by binding to it during the cell cycle, thus attenuating DNA replication. In human GBM, MGMT harbors a PCNA-interacting protein motif (PIP box), thus associating with PCNA and in turn, p21Cip1. PCNA is strictly controlled by p21Cip1, a strong binder and sequestrator of the former. Alkylating DNA damage induces MGMT and PCNA colocalization, and this occurs mainly in glioma cells deficient in p21Cip1, as p21Cip1 expression directly correlates with higher MGMT mRNA and protein levels, higher MGMT activity and greater resistance to alkylating agents. p21Cip1 strongly disrupts MGMT-PCNA complexes within glioma cells. MGMT proteins are downregulated at mid to late S-phase and specifically depredated by the PCNA-dependent ubiquitin-ligase, CRL4Cdt2.
As the p21Cip1 protein harbors binding domains for CDKs and PCNA, it serves as an upregulator of MGMT expression, enhancing its transcription and expression, thus providing glioma resistance to alkylating anticancer drugs.
MicroRNAs (miRs) are endogenous small non-coding RNAs which regulate gene expression post-transcriptionally. They are commonly deregulated in different types of cancer, including gliomas. In cancer cells, miR regulate cell viability, proliferation, differentiation, migration, invasion, apoptosis, chemoresistance and radioresistance. They can function as oncogenes by promoting cell growth or as anti-oncogenes by suppressing tumor growth.
Recent reports indicate a strict relationship between the p21Cip1 signaling pathway and miRs [Table 1]. The expression of miRs is significantly upregulated in gliomas as compared to that of adjacent non-tumor tissues or normal brain tissue. The expression of miR-10b, -17-92, -92b, -93, -149, -193b, 6798-3p was elevated in glioma tissues as compared to normal controls and significantly increased with tumor grade progression. It has been demonstrated that survival of GBM patients expressing high levels of miR-10 is lower in comparison with patients with low miR-10 levels indicating that miR-10 may contribute to glioma growth in vivo. miR-10b inhibition enhances the expression of direct miR-10b targets, namely BCL2L11/Bim, TFAP2C/AP-2γ, CDKN1A/p21, and CDKN2A/p16, thereby reducing glioma cell growth by cell-cycle arrest and apoptosis. In cells expressing high levels of p21Cip1, miR-10 represses E2F1-mediated transcription, leading to down-regulation of miR-15/16 and E2F1 target genes, thus delaying progression through to S-phase of the cell cycle. Consequently, miR-15/16 activities are reduced through the repression of many of its targets such as FBXW7, the ubiquitin ligase that destabilizes Cyclin E. However, miR-10b inhibition induces a weaker E2F1 response in GBM cells that express low levels of p21Cip1. Using the clustered regularly interspaced short palindromic repeats-cas9 system, it has also been demonstrated that ablation of the miR-10b gene, through alterations in the expression of several targets including p21Cip1, strongly reduces glioma cell viability in both in vitro and in vivo experimental models. This suggests miR-10b gene editing to be a promising therapeutic approach for permanent elimination of essential regulators of tumor survival.
miRs targeting p21Cip1 in glioma
|miR-7||ND||Pathological grade ↑|||
|miR-10b||Bim/AP-2/p16/p21Cip1||Proliferation, cycle arrest ↓|||
|miR-17-92||CTGF||Apoptosis ↑, proliferation ↓|||
|miR-92b||TGF/SMAD3||Viability, proliferation ↑|||
|miR-93||p21Cip1||Chemoresistance to Temozolomide ↑|||
|miR-139||ND||Pathological grade ↑|||
|miR-146b-5p||HuR/-catenin||Stemness, radioresistance ↑|||
|miR-149||Casp-2||Cell survival ↑|||
|miR-184||KT/NF-κB||Proliferation, invasion ↑|||
|miR-329||E2F1||Cell growth ↓|||
|miR-656||BMPR1A||Proliferation, migration/invasion neutrosphere formation ↓|||
|miR6798-3p||Ars2||Proliferation, tumorigenesis ↑|||
In addition, the miR-17-92 locus has been found to be amplified in GBM specimens. Inhibition of miR-17-92 activities enhances apoptosis and reduces cell proliferation of GBM spheroids. miR-17-92 inhibition has also been associated with increased expression of CTGF, a direct target of miR-17-92 in GBM spheroids, as well as CDKN1A, E2F1, PTEN.
Several target of miRs are represented by members of the TGF/SMAD pathways. In GBM tissues overexpressing miR-92b, the expression of SMAD3 was found to be reduced when compared with that of normal brain tissues. miR-92b directly affects SMAD3 expression by targeting the 3’-untranslated region. Silencing miR-92b inhibits GBM viability through upregulation of the TGF/SMAD3/p21Cip1 signaling pathway. Furthermore, in vivo treatment with miR-92b inhibitors was shown to reduce tumor growth, demonstrating the ability of miR-92b to act as an oncogene by promoting GBM cell proliferation. Therefore, miR-92b may be a future potential target for the development of miR-based therapies. miR-193b has also been shown to be upregulated in glioma patients, while its overexpression has been associated with poor prognosis. Downregulation of miR-193b correlates with decreased cell growth. SMAD3 is a direct target of miR-193b, and downregulation of SMAD3 attenuates miR-193b suppression of glioma proliferation. Thus, miR-193b regulates cell growth through the TGF-β pathway by modulating SMAD3.
Upregulation of miR-93 has also been demonstrated to be associated with advanced malignancy as it promotes the proliferation, migration and invasion of glioma cells. miR-93 regulates the cell cycle by controlling the p21Cip1, p27KIP1, p53 and cyclin D1 expression. Since p21Cip1 is a direct target of miR-93, p21Cip1 knockout attenuates the suppressive effects of miR-93 on cell cycle progression and colony formation. In addition, the chemosensitization of GBM cells to temozolomide is markedly increased when miR-93 is inhibited. Overexpression of miR-149 in gliomas augments pro-survival activity, inhibits apoptosis and induces xenografted tumor growth in vivo. Given that caspase-2 is a functional target of miR-149, its expression is inversely associated with miR-149 in vitro. miR-149 promotes cell survival in U87 and A172 glioma cell lines and targets caspase-2, through p53 and p21Cip1 inactivation.
Several miRs have be found to be down-regulated in glioma patients, thus functioning as tumor suppressor genes (e.g., miR-34a, -128, -184, -223-329 and -656). miR-34a maps to chromosome 1p36.23, a region often deleted in GBM. For this reason, the expression of miR-34a is lower in GBM samples as compared with that of normal brain tissue. In glioma patients, an miR-34a deletion is accompanied by the amplification of epidermal growth factor receptor (EGFR). Notably, mean survival time is shorter in GBM patients with EGFR amplification and miR-34a deletion. Moreover, enforced expression of miR-34a in GBM cells decreases migration and levels of cyclin-A1, -B1, -D1, and -D3, as well as cyclin-dependent kinases, while increasing the expression of cyclin kinase inhibitor proteins such as p21 or p27KIP1. In in vivo xenograft mouse model, the injection of U251 cells overexpressing miR-34a induced the development of smaller tumors compared with tumors derived from wild-type U251 cells. Since miR-34a targets Yin Yang-1, a transcription factor that stimulates the expression of EGFR, the expression of EGFR is reduced in cells overexpressing miR-34a. miR-34a act as a tumor suppressor by inhibiting the growth of GBM cells in vitro and in vivo.
miR-128 expression reduced glioma cell proliferation in vitro and in vivo in a glioma xenograft growth model. It reduced Bmi-1 oncogene expression, by direct regulation of the Bmi-1 mRNA 3’-untranslated region, through a miR-128 binding site. Relative to normal brain tissues, Bmi-1 is upregulated and miR-128 is down-regulated in glioma samples. Bmi-1 induces the silencing of several genes through epigenetic chromatin modifications. It is responsible for decreasing histone methylation [H3K27me(3)] and Akt phosphorylation, while upregulating p21Cip1 levels. Bmi-1 also promotes self-renewal of stem cells, while miR-128 [by down-regulating Bmi-1 levels] inhibits self-renewal of glioma. miR-184 levels are reduced in aggressive human tumor cells. Thus, a potential therapeutic strategy which targets miR-184 may prove useful in reducing cancer aggressiveness. miR-184 inhibits the proliferation and invasion of the U87MG cell line. It arrests the cell cycle and prevents adhesion by upregulating the expression of p53 and p21Cip1, increasing caspase-3/8 activity, suppressing SND1, MMP-2/9, CD44 expression, and the activity of the AKT/NF-κB pathway. Another miR involved in glioma development is miR-223. It represses nuclear factor I-A (NFIA) expression, a key regulator of gliogenesis. miR-223 and NFIA expression negatively correlate in human GBM tumors. The miR-223/NFIA axis suppresses tumorigenesis in human glioma cells. The NFIA factor directly represses p21Cip1, and is required for tumorigenesis in a mouse neural stem cell model of glioma.
miR-329, which is located on 14q32.31, is down-regulated in glioma and it is able to target E2F1. Its overexpression blocks G1/S transition in LN18 and T98G cell lines and suppresses cell proliferation as well as colony formation. It also decreases the phosphorylation of Akt and cyclin D1, inducing p21Cip1 upregulation and suppression of cell growth through the inhibition of the E2F1-mediated Akt pathway.
miR-656 was found to be downregulated in glioma. In human glioma tissues, expression of BMPR1A [a miR-656 target] is negatively correlated with miR-656 levels. miR-656 suppresses glioma cell proliferation, neurosphere formation, migration and invasion with or without exogenous BMP-2. Knockdown of BMPR1A diminished the antiproliferative effect of miR-656 in vitro. Moreover, the canonical BMP/SMAD pathways were shown to be inhibited by miR-656 overexpression. Several molecules, including cyclin B, cyclin D1, matrix metalloproteinase-9, p21Cip1 and p27KIP1 are involved in miR-656 functions within glioma cells. Ectopic expression of miR-656 reduced tumor size and prolonged the survival of mice, regardless of whether treatment with BMP-2 was administered.
Some miRs, even if down-regulated in gliomas, function as tumor promoters. For example, an important role has been described for miR-454, which is down-regulated in GBM primary tumors and cell lines. Overexpression of miR-454 in GBM cells resulted in the arrest at the G0/G1 phase, resulting in the inhibition of cell proliferation. 3-phosphoinositide-dependent protein kinase-1 (PDK1), one of miR-454 targets, was found to increase miR-454 levels, decrease PDK1 expression, down-regulate Cyclin D1 and upregulate p-Rb and p21Cip1.
In addition, arsenic resistance protein 2 (Ars2), a component of the nuclear RNA cap-binding complex, that is involved in miR biogenesis, proliferation and tumorigenicity, was found to be overexpressed in glioma cell lines. It is also associated with poorer overall survival in GBM patients. In fact, not only several miRs have been found to be associated with pathological grade, tumor development and stemness in glioma[50,51], but also it has been demonstrated that overexpression of Ars2 stimulates glioma cell proliferation and colony formation and colony formation. Knockdown of Ars2 reduces miR-6798-3p expression, causing p53 and p21Cip1 upregulation which leads to apoptosis. By using an orthotopic GBM xenograft model, knockdown of Ars2 was found to reduce tumor growth and to extend the survival time of tumor-bearing mice.
Long noncoding RNAs (LncRNAs) represent a class of long transcribed noncoding RNAs (ncRNA) molecules, which are longer than 200 nucleotides. They do not code for proteins and are involved in tumor development and progression. Long intergenic noncoding RNAs (LincRNAs) are long RNA transcripts which control cell differentiation and maintenance of cell identity. All of the above have recently been found to be altered in various cancer types [Table 2]. LncRNAs have been recognized as regulators involved in different steps of the tumorigenic process. In gliomas, the functions of most LncRNAs are not well known, and the mechanisms controlling the proliferation, invasion, angiogenesis, radiosensitivity or radioresistence, and GBM stemness remain poorly defined. While many LncRNAs have been identified, only a few have been functionally described in gliomas Moreover, CSC regulation by LncRNA following radiotherapy and the relationship between LncRNA and tumor spreading and radioresistance have been reported.
Long non-coding RNA and long intergenic non-coding RNA interacting with p21Cip1 pathway in glioma
|SNHG3||Epigenetic p21/KLE2 repression||Proliferation ↑ apoptosis ↓|||
|RP11-732M18.3||Binding to 14.3.3 b/a||Cell growth ↑|||
|SNHG16||Caspase 3/9 reduction||Proliferation and apoptosis ↑|||
|SNHG20||p21Cip1 silencing||Proliferation ↑|||
|PTENP1||SHG44 reduction||Proliferation and metastasis ↓|||
|FAH83H-AS1||Epigenetic p21Cip1 silencing||Poor prognosis ↑|||
|LOC441208||Binding to b-catenin||Cellgrowth ↑|||
|p21Cip1||Reduced HIF-1 autophagy||Radiosensitivity ↑|||
|p21Cip1||HuR upregulation||Stemness and radioresistance ↓|||
|p21Cip1||Binding to hnRNP-K||Apoptosis ↑|||
|ASEN||Binding to UPF1||Senescence ↓|||
|p21Cip1||CRF/CRFR1||Proliferation and invasion ↑|||
Recent studies have evidenced that LncRNAs control the transcription of genes involved in DNA Damage Response (DDR), the latter of which is strictly associated with radiosensitivity and repair capacity[55,56]. The majority of LncRNAs are overexpressed in tumor cells, hence their inhibition might represent a new therapeutic target for glioma treatment.
Among LncRNA, RP11-732M18.3 is highly overexpressed in glioma cells and functions as an oncogene by interacting with 14-3-3β/α to promote glioma growth.
Its overexpression has been associated with the proliferation of glioma cells and tumor growth both in vitro and in vivo. LncRNA RP11-732M18.3 stimulates the G1/S cell cycle transition and cell proliferation. It has been found that interaction of LncRNA RP11-732M18.3 with 14-3-3β/α increases the degradation of the p21Cip1 protein. In fact, by promoting the recruitment of ubiquitin-conjugating enzyme E2 E1 (UBE2E1) to 14-3-3β/α and with the binding of 14-3-3β/α with UBE2E1, LncRNA RP11-732M18.3 stimulates the degradation of p21Cip1. Similarly, LOC441204-LncRNA, has been shown to be upregulated in glioma cell lines. It promotes tumor cell growth by stabilizing the β-catenin pathway. LOC441204 can bind to β-catenin and prevent its degradation, thus resulting in downstream p21Cip1 repression and cdk4 activation that in turn enhances glioma cell proliferation. The LOC441204 knockdown suppressed tumor cell proliferation of glioma cell lines.
The expression of the small nucleolar RNA host gene 16, SNHG16-LncRNA was upregulated in gliomas. Knockdown of SNHG16 is associated with high p21Cip1 expression, poor proliferation and increased apoptosis. Lnc-SNHG16 was shown to inhibit p21Cip1 expression and caspase 3/9 activation, while increasing cyclinD1/B1 expression. The SHNG3 upregulation in glioma promoted cell proliferation, accelerated cell cycle progression and repressed apoptosis. SNHG3 facilitated the malignant progression of glioma through epigenetically repressing KLF2 and p21Cip1 via recruitment of the enhancer of zeste homolog 2 to the promoter of KLF2 and p21Cip1. Similarly, overexpression of SNHG6 promotes the malignant phenotype, while loss-of-function revealed that the silencing of SNHG6 inhibits glioma cell growth in a p21Cip1-dependent manner.
The LncRNA PTEN pseudogene-1 (PTENP1) has been described to be involved in the development and progression of several cancers. However, little is known about the molecular mechanism by which lncRNA PTENP1 affects the development and progression of gliomas. Overexpression of PTENP1 suppressed SHG44 expression as well as U251 cell proliferation, invasion and migration by inducing p21Cip1 expression while inhibiting p38 signaling.
Among LincRNA, the LincRNA-p21Cip1 is an intergenic LncRNA which resides on the chromosome 17, upstream of the p21Cip1gene. It was identified ten years ago as being one of the eleven LincRNAs that are increased in expression in response to p-53 induced DNA damage.
LincRNA-p21Cip1 is the downstream target of p53 and controls the cell cycle, damage of DNA and its repair process. The LincRNA-p21Cip1 suppresses the p53-dependent transcription activity and affects p53-repressed genes. It is required to trigger p53-dependent apoptosis of DNA damaged cells through physical association with the ribonucleoprotein K (hnRNP-K).
LincRNA-p21Cip1 also regulates the G1/S checkpoint of the cell cycle by modulating the expression of its neighboring gene p21Cip1 and mediating radiation-induced apoptosis[66,67]. LincRNA-p21Cip1 is a potent suppressor of GSC and works by triggering apoptosis through the NOXA pro-apoptotic gene. It negatively regulates β-catenin activity in GSCs. Downregulation of LincRNA-p21Cip1 in GSCs results in upregulation of Hu antigen R (HuR), and this is caused by miR-146b-5p down-regulation. Moreover, knockout of LincRNA-p21Cip1 or HuR was shown to increase GSC β-catenin expression, stemness and GSCs radioresistance. Previous findings also showed that LincRNA-p21Cip1 is a hypoxia-responsive lncRNA able to regulate the cell cycle and apoptosis. It has also been shown to be responsible for the Warburg effect in cervical cancer. Although hypoxia was found to increase LincRNA-p21Cip1 expression in U251MG glioma cell line, its functions in hypoxic glioma remain unknown. Knockdown of LincRNA-p21 induced G2/M phase arrest, promoted apoptosis, decreased cell proliferation and motility, and also reduced autophagy through the HIF-1/Akt/mTOR/P70S6K pathway. These findings are suggestive of a mechanism by which LincRNA-p21Cip1 enhances the radiosensitivity of hypoxic tumor cells.
The antisense FAM83H RNA1 (FAM83H-AS1) is known to be upregulated in glioma. Silencing of FAM83H-AS1 suppressed glioma proliferation and apoptosis. FAM83H-AS1 inhibits CDKN1A expression by recruiting EZH2 to the promoter of CDKN1A, thereby influencing cell cycle and proliferation.
LincRNAs also regulates cellular senescence. Thus, LincRNA-ASEN, which is expressed in prematurely senescent cells, has been shown to repress cellular senescence by reducing p21Cip1 expression, both during and post-transcription. The complex formed by the interaction between Linc-ASEN and UPF1 can suppress p21Cip1 transcription through the recruitment of Polycomb Repressive Complex 1 and 2 (PRC1 and PRC2) to the p21 locus. This prevents binding of the transcriptional activator p53 to the p21 promoter through histone modification.
The identification of glioma-related biomarkers is an urgent necessity as it would enable the development of new therapeutic approaches, while improving the ability to predict responsiveness to chemo- and radiotherapy. In this regard, several miRs including miR-10b and miR-92b have been suggested as targets for the development of miRNA-based therapies. Inhibition of miR-93 enhanced the chemosensitization of glioma cells to TMZ treatment, while miR-17-92 and its target CTGF have been suggested for use in differentiation-promoting therapy in the treatment of GSC. Conversely, the upregulation of miR-193band miR-93 are associated with advanced malignancy and represent negative prognostic factors. Ars-2, which reduces miR-6798 expression, was associated with poor overall survival in glioma patients.
Finally, Shen et al. have suggest that the miR effect in cancer may also depend on the p53 status of tumor cells. In glioma cell lines harboring wild-type p53, miR-149 showed pro-survival function, as it targeted caspase-2 via the inactivation of p53/p21Cip1 pathways, while functioning as a tumor suppressor in proliferation and invasion of glioma cell lines with p53 mutations. miR-149 also inhibited proliferation and migration of glioma cell lines with p53 loss, suggesting its distinct biological function within both p53 wild-type and p53 mutated glioma cells.
LncRNAs have recently emerged as crucial players in the p21Cip1 complex signaling network as they control glioma development and progression[71-73], the activation of GSCs, as well as radioresistance. LncRNAs aberrantly expressed in CSCs in different cancer types such as epatocarcinoma, are active participants in the major signaling pathways governing DDR, DNA repair, apoptosis and epithelial-mesenchymal transition[54,74]. It has been also reported that the deregulation of LncRNAs plays a role in cancer recurrence and prognosis. With increasing knowledge on the expression profile of LncRNAs in cancers, their potential roles as biomarkers in diagnosis and prognosis have been highlighted. Expression levels of LncRNAs are closely associated with the real tumor status. Additionally, LncRNAs are more sensitive and specific as compared to the other conventional markers. Furthermore, given that LncRNAs are released in body fluids, this suggests their potential to be utilized in a clinical setting as non-invasive biomarkers.
In gliomas, several dysregulated LncRNAs are involved in proliferation, radioresistance, metastasis and cancer stem cell properties. A strong correlation between high LncRNA expression and clinical parameters has been reported for LncRNA-SNHG20 in glioma patients. High LncRNA-SNHG20 has been associated with larger tumor size, larger extent of resection, more advanced grade (WHO classification), poorer survival status and a higher incidence of recurrence. High SNHG20 levels are predictive of poor prognosis and represent an independent potential prognostic biomarker for glioma patients. Similarly, overexpression of SNHG3, SNHG6 and FAH83H-AS1, which promote the malignant phenotype, represent a negative prognostic factors[60,61,70].
Given the up-regulated expression of LncRNAs in glioma patients, and the role in cancer development and progression, LncRNA inhibition (e.g., RPH, SNHG3, SNHG16, LOC441204)[57-60] would represent a novel diagnostic and therapeutic strategy in gliomas. Moreover, as LincRNA-p21Cip1 enhances the radiosensitivity of hypoxic tumor cells, it represents a valuable target for radiation therapy in glioma patients. Furthermore, in GSCs, the LncRNA-mediated effects also depend on the epigenetic regulation of genes, particularly via recruitment of the Polycomb repressor complex and by acting as competing endogenous RNAs for miRNAs targeting genes involved in stemness and radioresistance, mainly of the WNT/B-catenin and p21Cip1 pathways.
Finally, several miRs such as miR26a and LncRNAs such as Lnc-TALC have been found to promote MGMT expression and TMZ resistance in gliomas. However, at present no miRs or LncRNAs targeting p21Cip have been employed to modulate alkylating agent resistance in gliomas.
The functions of the vast majority of miRNAs, LncRNAs and LincRNAs encoded by mammalian genome remain largely unknown. However, recent studies indicate that these transcripts play vital roles not only in cellular physiology, but also in cancer pathology. In this regard, alternations in their regulation have been found to be implicated in the development and progression of glioma[53,79,80]. Complex cross-talk and interplay between miRNAs, Lnc/LincRNAs and p21Cip1 orchestrate the regulation of protein expression and functions in cancer cells. Our understanding of the functional roles and molecular mechanisms of miR and LncRNAs in glioma and GSC is still nascent. Several findings indicate that miRs targeting p21Cip1 and more recently LncRNAs signatures correlate with glioma malignancy grade, histological differentiation and prognosis[47,51,70]. Further in-depth investigation into miRs, LncRNAs and LincRNAs, their complex mechanisms of regulation, as well as their involvement in glioma genesis, metastatic spread and therapeutic resistance will be necessary in order to develop novel and effective diagnostic and therapeutic strategies against primary and secondary GBM tumors.
Supervised the work and wrote the manuscript: Santoni G
Contributed to the preparation of the subchapters: Morelli MB, Amantini C
Contributed to the preparation of figures and tables: Maggi F, Marinelli O
Provided critical revision of the manuscript: Nabissi M
Collaborated in the Drafting of the introduction and conclusion: Santoni MAvailability of data and materials
Not applicable.Financial support and sponsorship
This work was supported by PRIN 2017 and Fondazione Umberto Veronesi (Post-doctoral Fellowship 2019 to Morelli MB).Conflicts of interest
All authors declared that there are no conflicts of interest.Ethical approval and consent to participate
Not applicable.Consent for publication
© The Author(s) 2020.
1. Ostrom QT, Gittleman H, Stetson L, Virk SM, Barnholtz-Sloan JS. Epidemiology of gliomas. Cancer Treat Res 2015;163:1-14.DOIPubMed
2. Reni M, Mazza E, Zanon S, Gatta G, Vecht CJ. Central nervous system gliomas. Crit Rev OncolHematol 2017;113:213-34.DOIPubMed
3. Scherer HJ. A critical review: the pathology of cerebral gliomas. J Neurol Neurosurg Psychiatry 1940;3:147-77.DOIPubMedPMC
4. Morrison SJ, Kimble J. Asymmetric and symmetric stem-cell divisions in development and cancer. Nature 2006;441:1068-74.DOIPubMed
5. Qian X, Hulit J, Suyama K, Eugenin EA, Belbin TJ, et al. P21/CIP1 mediates reciprocal switching between proliferation and invasion during metastasis. Oncogene 2013;32:2292-303.DOIPubMedPMC
6. Pei XH, Xiong Y. Biochemical and cellular mechanisms of mammalian CDK inhibitors: a few unresolved issues. Oncogene 2005;24:2787-95.DOIPubMed
7. Romanov VS, Pospelov VA, Pospelova TV. Cyclin dependent kinase inhibitors (CKIs) play a crucial role in the regulation of the cell cycle in non transformed cells and are implicated in suppression of cell proliferation under stress conditions caused by growth factor deficiency. Biochemistry (Mosc) 2012;77:575-84.
8. Abbas T, Dutta A. p21 in cancer: Intricate networks and multiple activities. Nat Rev Cancer 2009;6:400-14.DOIPubMedPMC
9. Bertoli C, Skotheim JM, De Bruin RAM. Control of cell cycle transcription during G1 and S phases. Nat Rev Mol Cell Biol 2013;14:518-28.DOIPubMedPMC
10. Jung YS, Qian Y, Chen X. Examination of the expanding pathways for the regulation of p21 expression and activity. Cell Signal 2010;22:1003-12.DOIPubMedPMC
11. Mullan PB, Quinn JE, Harkin DP. The role of BRCA1 in transcriptional regulation and cell cycle control. Oncogene 2006;25:5854-63.DOIPubMed
12. Meng XM, Chung ACK, Lan HY. Role of the TGF-β/BMP-7/Smad pathways in renal diseases. Clin Sci 2013;124:234-54.DOIPubMed
13. Lee EW, Lee MS, Camus S, Ghim J, Yang MR, et al. Differential regulation of p53 and p21 by MKRN1 E3 ligase controls cell cycle arrest and apoptosis. EMBO J 2009;28:2100-13.DOIPubMedPMC
14. Xu H, Wang Z, Jin S, Hao H, Zheng L, et al. Dux4 induces cell cycle arrest at G1 phase through upregulation of p21 expression. BiochemBiophys Res Commun 2014;446:235-40.DOIPubMed
15. Hydbring P, Malumbres M, Sicinski P. Non-canonical functions of cell cycle cyclins and cyclin-dependent kinases. Nat Rev Mol Cell Biol 2016;17:280-92.DOIPubMedPMC
16. Wu S, CetinKaya C, Munoz-Alonso MJ, Von Der Lehr N, Bahram F, et al. Myc represses differentiation-induced p21/Cip1 expression via Miz-1-dependent interaction with the p21 core promoter. Oncogen 2003;22:351-60.DOIPubMed
17. Choi W Il, Kim MY, Jeon BN, Koh DI, Yun CO, et al. Role of promyelocytic leukemia zinc finger (PLZF) in cell proliferation and cyclin-dependent kinase inhibitor 1A (p21WAF/CDKN1A) Gene repression. J Biol Chem 2014;289:18625-40.DOIPubMedPMC
18. Zeng PY, Berger SL. LKB1 is recruited to the p21/WAF1 promoter by p53 to mediate transcriptional activation. Cancer Res 2006;66:10701-8.DOIPubMed
19. Lai D, Chen Y, Wang F, Jiang L, Wei C. LKB1 controls the pluripotent state of human embryonic stem cells. Cell Reprogram 2018;14:164-70.DOIPubMed
20. Koutsodontis G, Tentes I, Papakosta P, Moustakas A, Kardassis D. Sp1 plays a critical role in the transcriptional activation of the human cyclin-dependent kinase inhibitor p21WAF1/Cip1 gene by the p53 tumor suppressor protein. J Biol Chem 2001;276:29116-25.DOIPubMed
21. Kim HS, Heo JI, Park SH, Shin JY, Kang HJ, et al. Transcriptional activation of p21 WAF1/CIP1 is mediated by increased DNA binding activity and increased interaction between p53 and Sp1 via phosphorylation during replicative senescence of human embryonic fibroblasts. Mol Biol Rep 2014;41:2397-408.DOIPubMed
22. Karimian A, Ahmadi Y, Yousefi B. Multiple functions of p21 in cell cycle, apoptosis and transcriptional regulation after DNA damage. DNA Repair (Amst) 2016;42:63-71.DOIPubMed
23. Aasland D, Götzinger L, Hauck L, Berte N, Meyer J, et al. Temozolomide induces senescence and repression of DNA repair pathways in glioblastoma cells via activation of ATR-CHK1, p21, and NF-κB. Cancer Res 2019;79:99-113.DOIPubMed
24. Chen JF, Luo X, Xiang LS, Li HT, Zha L, et al. EZH2 promotes colorectal cancer stem-like cell expansion by activating p21/Cip1-Wnt/catenin signaling. Oncotarget 2016;7:41540-58.DOIPubMedPMC
25. Wen Y, Cai J, Hou Y, Huang Z, Wang Z. Role of EZH2 in cancer stem cells: from biological insight to a therapeutic target. Oncotarget 2017;8:37974-90.DOIPubMedPMC
26. Fan TY, Wang H, Xiang P, Liu YW, Li HZ, et al. Inhibition of EZH2 reverses chemotherapeutic drug TMZ chemosensitivity in Glioblastoma. Int J Clin ExpPathol 2014;7:6662-70.PubMedPMC
27. Xie Q, Wu Q, Mack SC, Yang K, Kim L, et al. CDC20 maintains tumor initiating cells. Oncotarget 2015;6:13241-54.DOIPubMedPMC
28. Morris-Hanon O, Furmento VA, Rodríguez-Varela MS, Mucci S, Fernandez-Espinosa DD, et al. The cell cycle inhibitors p21/Cip1 and p27Kip1 control proliferation but enhance DNA damage resistance of glioma stem cells. Neoplasia 2017;19:519-29.DOIPubMedPMC
29. Glaser T, Wagenknecht B, Weller M. Identification of p21 as a target of cycloheximide-mediated facilitation of CD95-mediated apoptosis in human malignant glioma cells. Oncogene 2001;20:4757-67.DOIPubMed
30. Head RJ, Fay MF, Cosgrove L, Fung K, Rundle-Thiele D, et al. Persistence of DNA adducts, hypermutation and acquisition of cellular resistance to alkylating agents in glioblastoma. Cancer Biol Ther 2017;18:917-26.DOIPubMedPMC
31. Happold C, Roth P, Wick W, Schmidt N, Florea AM, et al. Distinct molecular mechanisms of acquired resistance to temozolomide in glioblastoma cells. J Neurochem 2012;122:444-55.DOIPubMed
32. Mostofa A, Punganuru SR, Madala HR, Srivenugopal KS. S-phase specific downregulation of human O6-methylguanine DNA methyltransferase (MGMT) and its serendipitous interactions with PCNA and p21cip1 proteins in glioma cells. Neoplasia 2018;20:305-23.DOIPubMedPMC
33. Acunzo M, Romanoo G, Wernicke D, Croce CM. MicroRNA and cancer-a brief overview. Adv Biol Regul 2015;57:1-9.DOIPubMed
34. Gabriely G, Yi M, Narayan RS, Niers JM, Wurdinger T, et al. Human glioma growth is controlled by microRNA-10b. Cancer Res 2011;71:3563-72.DOIPubMedPMC
35. Teplyuk NM, Uhlmann EJ, Wong AH, Karmali P, Basu M, et al. MicroRNA-10b inhibition reduces E2F1-mediated transcription and miR-15/16 activity in glioblastoma. Oncotarget 2015;6:3770-83.DOIPubMedPMC
36. El Fatimy R, Subramanian S, Uhlmann EJ, Krichevsky AM. Genome editing reveals glioblastoma addiction to microRNA-10b. Mol Ther 2017;25:368-78.DOIPubMedPMC
37. Ernst A, Campos B, Meier J, Devens F, Liesenberg F, et al. De-repression of CTGF via the miR-17-92 cluster upon differentiation of human glioblastoma spheroid cultures. Oncogene 2010;29:3411-22.DOIPubMed
38. Wu ZB, Cai L, Lin SJ, Lu JL, Yao Y, et al. The miR-92b functions as a potential oncogene by targeting on Smad3 in glioblastomas. Brain Res 2013;1529:16-25.DOIPubMed
39. Zhong Q, Wang T, Lu P, Zhang R, Zou J, et al. miR-193b promotes cell proliferation by targeting Smad3 in human glioma. J Neurosci Res 2014;92:619-26.DOIPubMed
40. Chen R, Liu H, Cheng Q, Jiang B, Peng R, et al. MicroRNA-93 promotes the malignant phenotypes of human glioma cells and induces their chemoresistance to temozolomide. Biol Open 2016;5:669-77.DOIPubMedPMC
41. Shen X, Li J, Liao W, Wang J, Chen H, et al. microRNA-149 targets caspase-2 in glioma progression. Oncotarget 2016;7:26388-99.DOIPubMedPMC
42. Yin D, Ogawa S, Kawamata N, Leiter A, Ham M, et al. miR-34a functions as a tumor suppressor modulating EGFR in glioblastoma multiforme. Oncogene 2013;32:1155-63.DOIPubMedPMC
43. Godlewski J, Nowicki MO, Bronisz A, Williams S, Otsuki A, et al. Targeting of the Bmi-1 oncogene/stem cell renewal factor by microRNA-128 inhibits glioma proliferation and self-renewal. Cancer Res 2008;68:9125-30.DOIPubMed
44. Feng R, Dong L. Inhibitory effect of miR-184 on the potential of proliferation and invasion in human glioma and breast cancer cells in vitro. Int J Clin ExpPathol 2015;8:9376-82.PubMedPMC
45. Glasgow SM, Laug D, Brawley VS, Zhang Z, Corder A, et al. The miR-223/nuclear factor I-A axis regulates glial precursor proliferation and tumorigenesis in the CNS. J Neurosci 2013;33:13560-8.DOIPubMedPMC
46. Xiao B, Tan L, He B, Liu Z, Xu R. MiRNA-329 targeting E2F1 inhibits cell proliferation in glioma cells. J Transl Med 2013;11:172.DOIPubMedPMC
47. Guo M, Jiang Z, Zhang X, Lu D, Ha AD, et al. miR-656 inhibits glioma tumorigenesis through repression of BMPR1A. Carcinogenesis 2014;35:1698-706.DOIPubMed
48. Fang B, Zhu J, Wang Y, Geng F, Li G. MiR-454 inhibited cell proliferation of human glioblastoma cells by suppressing PDK1 expression. Biomed Pharmacother 2015;75:148-52.DOIPubMed
49. Chen Y, Hu X, Li Y, Zhang H, Fu R, et al. Ars2 promotes cell proliferation and tumorigenicity in glioblastoma through regulating miR-6798-3p. Sci Rep 2018;8:15602.DOIPubMedPMC
50. Koshkin PA, Chistiakov DA, Nikitin AG, Konovalov AN, Potapov AA, et al. Analysis of expression of micrornas and genes involved in the control of key signaling mechanisms that support or inhibit development of brain tumors of different grades. Clin Chim Acta 2014;430:55-62.DOIPubMed
51. Yang W, Yu H, Shen Y, Liu Y, Yang Z, et al. MiR-146b-5p overexpression attenuates stemness and radioresistance of glioma stem cells by targeting HuR/lincRNA-p21/B catenin pathway. Oncotarget 2016;7:41505-26.DOIPubMedPMC
52. Bhan A, Soleimani M, Mandai SS. Long non coding RNA and cancer: a new paradigm. Cancer Res 2017;77:3965-81.DOIPubMed
53. Wang L, Zhengwen H. Functional roles of long non-coding RNAs (LncRNAs) in glioma stem cells. Med SciMonit 2019;25:7567-73.DOIPubMedPMC
54. Chi HC, Tsai CY, Tsai MM, Yeh CT, Lin KH. Roles of long noncoding RNAs in recurrence and metastasis of radiotherapy-resistant cancer stem cells. Int J Mol Sci 2017;18:1903.DOIPubMedPMC
55. Engreitz JM, Ollikainen N, Guttman M. Long non-coding RNAs: Spatial amplifiers that control nuclear structure and gene expression. Nat Rev Mol Cell Biol 2016;17:756-70.DOIPubMed
56. Tannous BA, Teng J. Secreted blood reporters: insights and applications. BiotechnolAdv 2011;29:997-1003.DOIPubMedPMC
57. Kang CM, Bai HL, Li XH, Huang RY, Zhao JJ, et al. The binding of lncRNA RP11-732M18.3 with 14-3-3 β/α accelerates p21 degradation and promotes glioma growth. EBioMedicine 2019;45:58-69.DOIPubMedPMC
58. Lin TK, Chang CN, Tsai CS, Huang YC, Lu YJ, et al. The long non-coding RNA LOC441204 enhances cell growth in human glioma. Sci Rep 2017;7:5603.DOIPubMedPMC
59. Zhou XY, Liu H, Ding ZB, Xi HP, Wang GW. lncRNA SNHG16 exerts oncogenic functions in promoting proliferation of glioma through suppressing p21. Pathol Oncol Res 2020;26:1021-8.DOIPubMed
60. Fei F, He Y, He S, He Z, Wang Y, et al. LncRNA SNHG3 enhances the malignant progress of glioma through silencing KLF2 and p21. Biosci Rep 2018;38:BSR20180420.DOIPubMedPMC
61. Cai G, Zhu Q, Yuan L, Lan Q. LncRNA SNHG6 acts as a prognostic factor to regulate cell proliferation in glioma through targeting p21. Biomed Pharmacother 2018;102:452-7.DOIPubMed
62. Hu S, Xu L, Li L, Luo D, Zhao H, et al. Overexpression of lncRNA PTENP1 suppresses glioma cell proliferation and metastasis in vitro. Onco Targets Ther 2018;12:147-56.DOIPubMedPMC
63. Huarte M, Guttman M, Feldser D, Garber M, Koziol MJ, et al. A large intergenic noncoding RNA induced by p53 mediates global gene repression in the p53 response. Cell 2010;142:409-19.DOIPubMedPMC
64. Hall JR, Messenger ZJ, Tam HW, Phillips SL, Recio L, et al. Long noncoding RNA lincRNA-p21 is the major mediator of UVB-induced and p53-dependent apoptosis in keratinocytes. Cell Death Dis 2015;6:e1700.DOIPubMedPMC
65. Dimitrova N, Zamudio JR, Jong RM, Soukup D, Resnick R, et al. LincRNA-p21 activates p21 in cis to promote Polycomb target gene expression and to enforce the G1/S checkpoint. Mol Cell 2014;54:777-90.DOIPubMedPMC
66. Deng J, Yang M, Jiang R, An N, Wang X, et al. Long non-coding RNA HOTAIR regulates the proliferation, self-renewal capacity, tumor formation and migration of the cancer stem- like cell (CSC) subpopulation enriched from breast cancer cells. PLoS One 2017;12:e0170860.DOIPubMedPMC
67. Dutertre M, Lambert S, Carreira A, Amor-Guéret M, Vagner S. DNA damage: RNA-binding proteins protect from near and far. Trends BiochemSci 2014;39:141-9.DOIPubMed
68. Wang G, Li Z, Zhao Q, Zhu Y, Zhao C, et al. LincRNA-p21 enhances the sensitivity of radiotherapy for human colorectal cancer by targeting the Wnt/β-catenin signaling pathway. Oncol Rep 2014;31:1839-45.DOIPubMed
69. Shen Y, Liu Y, Sun T, Yang W. LincRNA-p21 knockdown enhances radiosensitivity of hypoxic tumor cells by reducing autophagy through HIF-1/Akt/mTOR/P70S6K pathway. Exp Cell Res 2017;358:188-98.DOIPubMed
70. Bi YY, Shen G, Quan Y, Jiang W, Xu F. Long noncoding RNA FAM83H-AS1 exerts an oncogenic role in glioma through epigenetically silencing CDKN1A (p21). J Cell Physiol 2018;233:8896-907.DOIPubMed
71. Li XS, Shen FZ, Huang LY, Hui L, Liu RH, et al. lncRNA small nucleolar RNA host gene 20 predicts poor prognosis in glioma and promotes cell proliferation by silencing P21. Onco Targets Ther 2019;12:805-14.DOIPubMedPMC
72. Lee HC, Kang D, Han N, Lee Y, Hwang HJ, et al. A novel long noncoding RNA Linc-ASEN represses cellular senescence through multileveled reduction of p21 expression. Cell Death Differ 2020;27:1844-61.DOIPubMedPMC
73. Yang J, Gan X, Tan B, Wang J, Chen Y. Corticotropin-releasing factorsuppresses glioma progression by upregulation of long non-coding RNA-p21. Life Sci 2019;216:92-100.DOIPubMed
74. He Y, Meng XM, Huang C, Wu BM, Zhang L, et al. Long noncoding RNAs: novel insights into hepatocelluar carcinoma. Cancer Lett 2014;344:20-7.DOIPubMed
75. Schmitt AM, Chang HY. Long noncoding RNAs in cancer pathways. Cancer Cell 2016;29:452-63.DOIPubMedPMC
76. Heery R, Finn SP, Cuffe S, Gray SG. Long non-coding RNAs: key regulators of epithelial-mesenchymal transition, tumour drug resistance and cancer stem cells. Cancers (Basel) 2017;9:38.DOIPubMedPMC
77. Huang W, Zhong Z, Luo C, Xiao Y, Li L, et al. The miR-26a/AP-2α/Nanog signaling axis mediates stem cell self-renewal and temozolomide resistance in glioma. Theranostics 2019;9:5497-516.DOIPubMedPMC
78. Wu P, Cai J, Chen Q, Han B, Meng X, et al. Lnc-TALC promotes O 6-methylguanine-DNA methyltransferase expression via regulating the c-Met pathway by competitively binding with miR-20b-3p. Nat Commun 2019;10:2045.DOIPubMedPMC
79. Zhang Y, Dutta A, Abounader R. The role of microRNAs in glioma initiation and progression. Front Biosci 2012;17:700-12.DOIPubMedPMC
80. Sana J, Hajduch M, Michalek J, Vyzula R, Slaby O. MicroRNAs and glioblastoma: roles in core signalling pathways and potential clinical implications. J Cell Mol Med 2011;15:1636-44.DOIPubMedPMC
Santoni G, Morelli MB, Nabissi M, Maggi F, Marinelli O, Santoni M, Amantini C. Cross-talk between microRNAs, long non-coding RNAs and p21Cip1 in glioma: diagnostic, prognostic and therapeutic roles. J Cancer Metastasis Treat 2020;6:22. http://dx.doi.org/10.20517/2394-4722.2020.49
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