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From: Multiplexed bioluminescence imaging of cancer cell response to hypoxia and inflammation in the caudal-artery injection model of bone metastasis during zoledronic acid treatment

Multiplexed bioluminescence imaging of cancer cell response to hypoxia and inflammation in the caudal-artery injection model of bone metastasis during zoledronic acid treatment

Figure 1. Construction of the Cluc-GPI reporter. A: Schematic of Cluc-GPI. Cluc is fused with the C-terminal domain of CD90; B: Intracellular (Cell) and extracellular (Medium) Cluc bioluminescence intensities were measured 24 h after transfection of the reporter gene. n = 3, *P < 0.05; C: Cell lysates of HeLa transfected with Fluc, Rluc or Cluc-GPI reporter genes were measured for bioluminescence intensities after reactions with the indicated luciferase substrates. n = 3, *P < 0.05. Cluc: Cypridina luciferase; Fluc: Firefly luciferase; Rluc: Renilla luciferase; GPI: glycosylphosphatidylinositol