fig1

Figure 1. EpCAM+CK+CD45- tumor cells detected by flow cytometry by first gating out cell debris and cell clumps in forward/side scatter plot (A); threshold for specific EpCAM (APC) and CK (FITC) signals determined using sample stained with isotype control antibodies. Gates set to have no positive events above these thresholds in control sample (B); representative histograms shown of individual criteria gated on (C-E) Jurkat cells and (F-H) BT-474 human tumor cells