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Figure 4. GPR81 regulates lactate uptake in MCF-7 epithelial breast cancer cell lines. A: intracellular lactate of MCF-7-siNT (control) and MCF-7-siGPR81 measured using NMR analysis after 72 h of transfection; B: lactate concentration in the medium of MCF-7-siNT 10 nmol/L and MCF-7-siGPR81 10 nmol/L were measured after 96 h of transfection; C: glucose consumption rate of MCF-7-siNT and MCF-7- siGPR81 were measured after 96 h of transfection. MCF-7-siNT and MCF-7-siGPR81 cells were cultured and transfected in 3-dimensional Matrigel with physiological modified medium and measurements were taken after 24 h of culture in fresh medium. The bars represent the mean ± S.E.M of three independent experiments; *P < 0.05; **P < 0.01; (ordinary one-way ANOVA)