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Figure 4. Osteolytic lesions, trabecular bone volume and the number of osteoclasts at the bone-tumor interface. Osteolytic lesion area was analyzed from X-ray images of both hind limbs, trabecular bone area and the number of osteoclasts in the bone-tumor interface in midsagittal histological sections of the left hind limb. A: Representative images showing established osteolytic lesions before treatment and at sacrifice; B: osteolytic lesion area was decreased in the ZOL and DOX + ZOL groups as measured by radiography; C: trabecular bone area was increased in the group receiving only ZOL, but the effect was not statistically significant in the DOX + ZOL group. Data are expressed as mean ± SD, n = 8 animals. Scale bars 2 mm, arrows osteolytic lesions. **Significantly different from control group (P < 0.01); ***significantly different from control group (P < 0.001); NS: non-significant; DOX: doxorubicin; ZOL: zoledronic acid