fig2

Figure 2. Representative immunofluorescence immunohistochemical-stained sections of liver metastasis from colon adenocarcinoma demonstrating the expression of pro-renin receptor [(A), red] by the cells within the tumor nests (TNs), the cells within the peritumoral stroma (PTS) regardless of whether they were OCT4⁺ [(A), green] or OCT4- (A). Angiotensin converting enzyme [(B), green] was expressed on the endothelium of the microvessels within the PTS, which also expressed SOX2 [(B), green]. Angiotensin II receptor 1 (ATIIR1) [(C), green] was demonstrated on cells within the TNs, the endothelium of the microvessels within the PTS (C) and the cells within the PTS (C), which also expressed SOX2 [(C), red]. A similar expression pattern was seen for ATIIR2 [(D), red] in the cells within the TNs and the cells within of the PTS whether they expressed OCT4 [(D), green] or not (D). All slides were counter-stained with 4’,6’-diamino-2-phenylindole [(A-D), blue]. Scale bars: 20 μm; insert: 400 × magnification