fig1

Figure 1. Representative DAB IHC-stained sections of metastatic melanoma to the brain showing endothelial staining of ACE (A, brown), and mostly cytoplasmic staining of PRR (B, brown), ATIIR1 (C, brown) and ATIIR2 (D, brown). Nuclei were counter-stained with hematoxylin (A-D, blue). Original magnification: 200×. DAB: 3, 3-diaminobenzidine; IHC: immunohistochemical; ATIIR1: angiotensin II receptor 1; ATIIR2: angiotensin II receptor 2; PRR: pro-renin receptor; ACE: angiotensin converting enzyme