fig2

Figure 2. Representative IF IHC-stained sections of metastatic melanoma to the brain demonstrating cytoplasmic expression and some nuclear expression of PRR (A, red) on the OCT4⁺ (A, green) cells. ACE (B, green) was expressed by the ERG⁺ (B, red) endothelium of the microvessls. Some NANOG⁺ (C, red) cells expressed ATIIR1 (C, green). ATIIR2 (D, red) was expressed in the cytoplasm of the OCT4⁺ (D, green) cells. Cell nuclei were counterstained with 4’, 6’-diamidino-2-phenylindole (A-D, blue). Scale bars: 20 μm. IF: immunofluorescence; IHC: immunohistochemical; PRR: pro-renin receptor; ATIIR1: angiotensin II receptor 1; ATIIR2: angiotensin II receptor 2; ACE: angiotensin converting enzyme