fig5

Figure 5. EGFR and EGFR-signaling is downregulated in paxillinS178A mutant cells. A: bar graph displaying the 10 most down- and up-regulating genes in mutant cells when compared with wt cells (a); and Enrichr analysis using the GO Biological process function (processes are sorted by combined score) (b); B: Enrichr analysis using the PPI Hub proteins function (a), EGFR being the first PPI with the highest score (b); C: Western blot analysis confirms a decreased EGFR expression in all S178A clones. Ratios of EGFR levels relative to wt1 clone are indicated below the blot. EGFR downstream signaling towards Akt, ERK and JNK is affected in S178A clones; D: activity of AP-1 member c-Jun was reduced in paxillinS178A cells as determined by TATA-luciferase reporter assay