fig1

Figure 1. Increases in migration of MCF-7 human breast cancer cells following prior long-term exposure to several estrogen disrupting chemicals contained in personal care products. Cell migration was measured in real-time as electrical impedance (cell index) using xCELLigence technology with uncoated CIM-16 plates. Prior to the assay, cells had been grown. A: for 20 weeks with no addition, 5 × 10^–4 M methylparaben or 10^–5 M n-butylparaben; B: for 23 weeks with no addition, 10^–8 M 17β-estradiol, 10^-5 M octylmethoxycinnamate or 10^-5 M benzophenone-3; C: for 32 weeks with or without 10^-4 M aluminium chlorohydrate; D: for 9 weeks with or without 10^-7 M triclosan. Conditions were as published for the parabens^[30], UV filters^[35], and aluminium chlorohydrate^[42]. Data for triclosan are unpublished personal results. UV: ultraviolet