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Figure 2. ROR2 regulated the survival of LM8 in lung capillaries. A: fluorescently labeled LM8 cells in the lungs were quantified using an inverted confocal fluorescence microscope (top). Data are shown as the mean ± SD of three fields of fluorescently labeled cells in each lung on the indicated days. n = 3, **P < 0.01. Representative image of LM8 cells in lungs on the indicated days (bottom). Red: blood vessel, Green: tumor cell, Black: lung tissue. Scale: 50 µm; B: ROR2 protein level in the indicated LM8 sublines. The numbers below the bands indicate the corresponding ROR2 expression levels relative to GAPDH; C: fluorescence intensity in lung lysates prepared from mice injected with the indicated LM8 sublines was quantified on day 0 (30 min) and day 2 (48 h). Data are shown as the ratio of day 2 fluorescence intensity normalized to day 0. n = 3, *P < 0.05. ROR2: receptor tyrosine kinase-like orphan receptor 2; LM8-H: LM8 cell line with high metastatic ability; LM8-L: LM8 cell line with low metastatic ability; H/Ror2-KO: LM8-H knocked out of Ror2; L/ROR2: LM8-L expressing ROR2; KO/ROR2: H/Ror2-KO expressing ROR2; n.s: not significant