fig1

Figure 1. Effects of oestradiol on mouse tibiae. Eight-week-old BALB/c nu/nu mice received a 28-day release 1.7 mg 17β oestradiol pellet via subcutaneous implant or 12 mg/L 17β oestradiol continually via their drinking water (n = 10/group). Mice were culled after 28 days and serum and tibiae isolated for analysis: (A) mean ± SEM circulating concentrations of 17β oestradiol as measured by ELISA; (B) effects of 17β oestradiol on mean ± SEM for percentage of trabecular bone volume compared with tissue volume (BV/TV%) in the proximal tibiae; (C) representative, three-dimensional micro-CT images of tibiae from control (untreated) and 17β-treated tibiae. Statistical analysis was by student t-test. **P < 0.01; ***P < 0.001. Data shown are previously unpublished observations from the Ottewell laboratory.