fig1

Figure 1. Effect of canine prostatic cancer cell conditioned media (CM) on bone formation and bone resorption in vitro. (A) Bone formation was visualized by calcein labeling of mineralization. Representative images of 1.5 mm calvarial disks (parietal bone). (B) The total fluorescence intensity was quantified and the fold increase over control was measured (5 pairs for each). (C) ALP activity in control calvaria and calvaria treated with canine PCa CM. (D) Amount of calcium present in the media for 2 days of culture. Data were expressed as mean ± SD. Significant differences were marked with asterisks (*P < 0.05, **P < 0.01, ***P < 0.001). CM: Conditioned medium; ALP: alkaline phosphatase.