fig1

Figure 1. VISTA expression in the periphery versus the tumor microenvironment. BALB/c mice were injected intradermally with 100k CT26 cells and were grown for two weeks. Tumors and spleens were dissociated and processed for flow cytometry. Cells from VISTA-deficient mice were used for staining controls. (A) Overlays showing VISTA expression on CD8+ T cells in the spleen or tumor. (B) VISTA expression as Mean Fluorescence intensity in spleen versus tumor within the indicated cell subsets. Data are represented as mean ± SD. VISTA: V-domain Ig Suppressor of T cell Activation.