Type: Original Article
Title: Cellular signaling pathways activated by α-Mangostin during induction of cell death in human esophageal adenocarcinoma
Authors: S. Balakrishna Pai
Affiliations: Director of Instructional Laboratories，Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology & Emory University.
Abstract: Aim: Alpha mangostin, a xanthone from mangosteen fruit, has been reported to have beneficial effects against various cancers. However, the potential of this molecule against esophageal adenocarcinoma has not been investigated in detail. The current study sought to investigate the cytotoxic effect of α-mangostin on the esophageal carcinoma cell line OE33 as well as elucidate the mechanism of action.
Methods: A Cell Counting Kit-8 assay was used to assess the cell viability after treatment with α-mangostin. Additionally, multicaspase, cell cycle, and mitopotential flow cytometric assays were also performed to further assess the contribution of various signaling pathways on α-mangostin-induced cell death of OE33 cells.
Results: Cell Counting Kit-8 assay indicated that cell viability was significantly reduced in a dose-dependent manner after 72 hours of α-mangostin treatment. After assessing the cells via an Annexin V & Dead Cell Kit at 24, 48, and 72 hours, it was determined that OE33 cells underwent early apoptosis after only 24 hours of treatment at a concentration of 15 µM. α-mangostin treatment did not significantly change the mitochondrial potential nor did it impact the cell cycle progression. Further, detailed analysis of apoptosis signaling pathway indicated that caspase cascade could be responsible for reduction in cell viability of treated cells. α-mangostin induces apoptosis at an early timepoint in OE33 cells and does so at a relatively low concentration.
Conclusion: Taken together, data from the current study, in addition to deciphering the signaling pathways, highlight the potential therapeutic potential of α-mangostin against esophageal adenocarcinoma.