fig2

Figure 2. Withaferin A reduced side-population and cell aggregates in UP-LN1 cells. (a) Withaferin A reduced the percentage of side-population cells in UP-LN1 in a dose-dependent manner as indicated by our cytofluorometric analysis. The reduction of side-population cells in different populations of UP-LN1 cells was plotted against the concentration of withaferin A (right panel); (b) sulforhodamine B viability assay indicated that withaferin A targeted floating (F) cells most effectively and also in a dose-dependent manner as compared with adherent (A) or parental (P) cells. The difference between F and A cells is signifi cant (P < 0.01), and so is the difference between F and P cells (P < 0.01) at each of the three withaferin A dose levels (5, 10, 25 μmol/L) tested; (c) microscopic analysis of F cells under the influence of withaferin A demonstrated a significant reduction in the formation of F-cell aggregates or spheres. At 10 μmol/L, withaferin A reduced F-cell aggregates by approximately 80%