fig5

Figure 5. Cell cycle analysis showing that curcumin causes greater migration of QLCs from the G₀/G₁ phase to other stages of the cell cycle: QLCs were treated with curcumin (10 µg/mL) for 48 h. After 48 h the quiescent cells were incubated with 5-FU (6 µg/mL) for another 24 h and then subjected to PI staining. The stained cells were acquired and analyzed using BD FACSCalibur^TM. As compared to the vehicle control (VC), a much higher percentage of cells treated with curcumin were pushed into the S and G₂/M phase. More importantly, the QLCs that were exposed to curcumin were more effectively killed by 5-FU than cells that were not. (A) Side scatter plot of untreated vehicle control cells (QLCs), where R1 is the gated population of QLCs that is positive for PI. The histograms demonstrate a distinct pattern of the different phases of the cell cycle marked as M1 (G₁/G₀ phase), M2 (S-phase), M3 (G₂/M phase) and M4 (Dead cells) of untreated QLCs (VC) and of QLCs subjected to curcumin and/or 5-FU treatments; (B) a tabular and graphical representation of , depicting the percentage of gated QLCs in each stage of the cell cycle in response to treatment with cucrumin and/or 5-FU