fig6

Figure 6. Combination therapy promotes higher caspase-3 activity in quiescent leukemic cells. QLCs were treated as represented in the graph. The treated cells were harvested and subjected to caspase-3 assay. Intensity of the color was measured at 400/405 nm by using microplate spectrophotometer. In the graph, VC represents vehicle control; 10CU represents the cells treated with curcumin only (10 µg/mL); 5-FU represents cells treated with 5-FU only (6 µg/mL), and 10CU+5-FU represents combination treatment of 10 µg/mL of curcumin and 6 µg/mL of 5-FU. Serum-starved KG1a cells treated with 10 µg/mL of curcumin induce minimum caspase-3 activity as compared to VC. Cells treated with a combination of 10 µg/mLof curcumin and 6 µg/mL of 5-FU induces maximum caspase-3 activity as compared to the cells exposed to only 5-FU. The data represent mean ± S.E.M of three independent experiments (* P ≤ 0.05, *** P ≤ 0.001)