fig7

Figure 7. Combination treatment with low curcumin concentrations and 5-FU leads to higher generation of NO in quiescent KG1a cells: QLCs were subjected to various treatments as shown in the graph. After treatment, the cells were spun, supernatant was collected into fresh plates and to the supernatant was added an equal volume of Griess reagent. The plate was incubated for 15 min in dark at room temperature (RT) and the intensity of color was measured at 540 nm using microplate spectrophotometer. PC represents positive control, that is sodium nitrite solution (50 µm). VC represents vehicle control; 10CU and 20CU represent the concentrations of curcumin used, that is cells treated with only 10 µg/mL and 20 µg/mL respectively; 5-FU represents cells treated with only 6 µg/mL of 5-FU; 10CU + 5-FU, and 20CU + 5-FU represents cells treated with combinatorial treatment of curcumin (10 µg/mL or 20 µg/mL) and 5-FU (6 µg/mL). Serum-starved KG1a cells treated with 10 µg/mL and 20 µg/mL of curcumin generated minimum amount of nitrites as compared to VC, whereas cells treated with a combination of 10 µg/mL or 20 µg/mL of curcumin and 6 µg/mL of 5-FU, respectively, showed maximum levels of nitrite as compared to the cells treated with only 5-FU. The data represents mean ± S.E.M of three independed experiments (* P ≤ 0.05, ** P ≤ 0.01)