fig8

Figure 8. QLCs subjected to combination treatment generate higher levels of ROS: Quiescent cells generated from KG1a cells were incubated with low concentrations of curcumin only (10 µg/mL and 20 µg/mL), 5-FU only (6 µg/mL) and a combination of curcumin and 5-FU, as shown in the graph. The treated cells were collected and spun, and the cell pellet was incubated with 100 µL of DCFDA (10 µm) for 30 min at 37°C. After incubation the cells were centrifuged, and the pellet was resuspended in 1 × PBS. The fluorescence was measured at 495-529 nm. In the graph, PC represents positive control that is hydrogen peroxide (50 µm), VC represents vehicle control; 10CU and 20CU are cell samples treated with curcumin only (10 µg/mL and 20 µg/mL, respectively), 5-FU represents 5-FU only (6 µg/mL) treated cells, and 10CU + 5-FU, 20CU + 5-FU represents combination treatment of curcumin (10 µg/mL or 20 µg/mL) and 5-FU (6 µg/mL), respectively. Quiescent KG1a cells treated with only 10 µg/mL or 20 µg/mL of curcumin generated minimum amount of ROS, as compared to VC. QLCs treated with a combination of 10 µg/mL or 20 µg/mL curcumin, respectively, and 6 µg/mL of 5-FU showed maximum ROS generation as compared to the cells treated with only 5-FU. The data represent mean ± S.E.M of three independent experiments (* P ≤ 0.05, ** P ≤ 0.01)