fig2

Figure 2. A combination of radiation treatment and Vorinostat has varied effects on viability and colony forming ability in a panel of cell lines and primary prostate epithelial cells. PNT1a, BPH-1, P4E6 and PC3 cells were treated with Vorinostat (10 µmol/L) or radiation (75 Gy) or both and measured using alamar blue assay at 24 h (A), 48 h (B) and 72 h (C) post-treatment. Primary epithelial cultures from six patients, normal (2 samples) (D) and prostate cancer (4 samples) (E) were treated with Vorinostat (10 µmol/L) or radiation (75 Gy) or both and measured using alamar blue assay at 24 h post-treatment. Each symbol represents a different patient sample; (F) primary epithelial cultures from four patients were treated with 2 Gy radiation or three concentrations of Vorinostat (low: 0.625 µmol/L, medium: 2.5 µmol/L, high: 10 µmol/L) or both and assessed for colony forming ability 10-14 days after growth. Colony forming ability is presented as % surviving fraction