fig4

Figure 4. Label-free quantitative phase imaging (QPI) shows that primary prostate cultures divide less frequently than cell lines but undertake significantly more movement in 2D culture. A panel of prostate cell lines was grown alongside a primary prostate epithelial culture in a 6-well dish and time-lapse imaging was carried out. (A) Brightfield images of each cell type; (B) QPI images of each cell type with cell segmentation outlines (colored lines) and cell tracking ID (colored numbers) shown; (C) 2D representation of tracking of each cell type (X-axis, x position; Y-axis, y position); (D) 3D representation of tracking of each cell type (as for 2D but including a Z-axis, time); (E) close up view of the 3D rendition showing the trace of two cells spinning round each other; (F) mean cell area is plotted for each cell type. Each dot represents a single cell track; (G) mean speed is plotted for each cell type. Each dot represents a single cell track; (H) the total dry mass of each frame of the time-lapse video is plotted, which is indicative of cell growth and proliferation