fig5

Figure 5. Signatures of two populations of cells within primary prostate cultures can be characterized from quantitative phase imaging (QPI) data and used to identify different cell populations within heterogeneous cultures. (A) Immunofluorescence of CD49b in a mixed culture of cells, transit amplifying (TA) cells and committed basal (CB) cells; (B) flow cytometry of TA and CB cells using the CD49b surface marker; (C) QPI images of TA and CB cells showing cell segmentation outlines (colored lines). Data from QPI analysis of each cell type was measured including (D) mean cell dry mass, (E) mean cell area and (F) cell sphericity; (G) analysis of a mixed culture of cells with gates applied to separate out the two cell populations on the basis of cell area. Data from the whole population (WP) and each cell type was measured and plotted as mean cell area and mean cell dry mass